Chimeric sheets of epithelial cells

Details Chimeric sheets of epithelial cells Chimeric sheets of epithelial cells

1993-01-21

1997-03-11

Guzo, David

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

424 931, 424 937, 435325, 435346, 435354, 623 15, C12N 506, A61F 210

Patent

active

056100078

ABSTRACT:
A process for culturing and generating a chimeric cell culture, in particular chimeric epithelium, is disclosed. The chimeric epithelium can be used to treat skin trauma such as burn victims. Autologous epithelial grafts have been used on burn patients although this requires that the patient's cells are cultured and expanded in vitro which generally takes four to five weeks. The chimeric epithelium of the present invention is composed of cells that are both autologous and allogeneic to the host. Therefore, the allogeneic cells can be maintained in a cell bank and co-cultured with autologous host cells when needed. This significantly reduces the time required (by up to 50%) for autologous cell expansion and culture prior to grafting. Furthermore, it has been demonstrated that the allogeneic cells are passively eliminated from the graft without rejection of the total graft.

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