Method to solubilize tissue plasminogen activator

Details Method to solubilize tissue plasminogen activator Method to solubilize tissue plasminogen activator

1987-12-10

1990-02-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435219, 424 9464, C12N 964, C12N 948, C12N 950, A61K 37547

Patent

active

048988260

ABSTRACT:
Practical amounts of tissue plasminogen activator (tPA) whether secreted by cells naturally producing it or prepared by recombinant means can be solubilized by providing, in aqueous medium at pH 5-14 8 a solubilizing amount of a basic amino acid optionally and preferably, in the presence of a citric acid moiety. The ability to solubilize tPA at concentrations of up to 50 mg is significant as an aid in permitting smaller volumes for purification and permitting single injections of the drug as opposed to intravenous administration.

REFERENCES:
patent: 4568544 (1986-02-01), Hasegawa et al.
patent: 4777043 (1988-10-01), Bennett et al.
Rijken et al., (1979) Biochi. Biophys. Acta 580:140-153.
Pennica et al., (1983) Nature 301:214-221.

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