Apparatus for separating mononuclear cells from blood and method

Details Apparatus for separating mononuclear cells from blood and method Apparatus for separating mononuclear cells from blood and method

1989-02-02

1990-09-04

Rosen, Sam

Liquid purification or separation

Processes

Separating

210767, 210789, 424529, 424532, 424534, 435 2, 435287, 435296, B01D 3302, B04B 300, A61K 3514

Patent

active

049542648

ABSTRACT:
A device for separating mononuclear cells from blood includes a collection tube, a first layer of a liquid density gradient material placed in the tube, a second layer of a gel-like substance placed in the tube and situated above the first layer, a porous foam member placed in the tube and situated above and in contact with the second layer of gel-like substance, and a third layer of a Newtonian gel-like substance placed in the tube above the porous foam member. The gel-like substance of the second layer has a lower specific gravity than that of the liquid density gradient material of the first layer so that it floats on top of the first layer. The gel-like substance of the second layer becomes less viscous when heated such that it is absorbed by the porous foam member and forms a hydraulic barrier between the liquid density gradient material of the first layer and a blood sample placed in the collection tube.

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