Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1987-06-18
1989-07-11
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436501, 436518, 436524, 436525, 436528, 436531, 436806, C12Q 168
Patent
active
048471933
ABSTRACT:
A method for detecting a first member of a specific binding pair in a sample, the first member being capable of forming a complex with a second member of the specific binding pair, includes immobilizing the second member on a surface of a piezoelectric oscillator, determining a first vibrational frequency of the oscillator on which the second member has been immobilized, contacting the surface of the oscillator on which the second member has been immobilized with the sample under conditions permitting formation of the complex, and with a particle under conditions permitting binding of the particle with the first member, mass coupling the bound particle with the oscillator, and determining a second vibrational frequency of the oscillator with which the particle has been mass coupled, the difference between the first vibrational frequency and the second vibrational frequency providing a measure of the quantity of the first member in the sample.
REFERENCES:
patent: 4236893 (1980-12-01), Rice
patent: 4242096 (1980-12-01), Oliveira et al.
patent: 4314821 (1982-02-01), Rice
patent: 4672040 (1987-06-01), Josephson
patent: 4735906 (1988-04-01), Bastiaans
"Piezoelectric Sorption Detector", William H. King, Jr., vol. 26, No. 9, Aug. 1964, Analytical Chemistry.
"An Immunospecific Microbalance", Shons et al., Journal of Biomedical Materials, vol. 6, pp. 565-570 (1972).
"Attachment of Nucleic Acids to Piezoelectric Crystals for Detection of Hybridization by Change in Mass", Flowers et al., Chemistry of DNA and RNA (204-207).
Bach David T.
Richards James C.
Gene-Trak Systems
Warden Robert J.
Wieder Stephen C.
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