Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-05-14
1988-11-22
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 56, 422 57, 422 58, 422 60, 422 61, 422102, 435175, 435805, 435810, 436503, 436525, 436527, 436530, 436531, 436823, 436824, G01N 5300, G01N 2100, G01N 33552
Patent
active
047865946
ABSTRACT:
A method is disclosed for determining an analyte in a sample suspected of containing the analyte. The analyte is a member of a specific binding pair (sbp) consisting of ligand and complementary receptor. The method comprises combining in an aqueous medium (1) said sample, (2) a first reagent comprising a non-dispersed surface to which is bound an sbp member that becomes bound to the surface in relation to the amount of analyte in the sample. The volume of the aqueous medium containing the above reagents is sufficiently large to allow complete immersion of the first reagent therein and determination of the analyte but not so large to result in substantial interference in the determination upon addition of a second reagent reactive with the conjugate of enzyme and sbp member and capable of generating a signal. The second reagent is present in a medium of sufficient volume to substantially increase the volume of the liquid medium and reduce interference in the determination. The second reagent is added to the first liquid medium without separation of the first reagent therefrom. The first reagent of the medium is examined for the presence of the signal. The present method avoids a separation and washing step normally found in separation assays. Thus, the present invention is less time consuming and less labor intensive. An apparatus for performing the above assay comprises a vessel having at least one vertical side wall, an open end and a single closed end generally opposite the open end. A discontinuously narrowed portion is adjacent the closed end and defines a well. A ledge depends from a vertical side wall adjacent the narrowed portion. Preferably, the vessel and the narrowed portion are cylindrical. A kit for performing the assay method of the invention is also described.
REFERENCES:
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 4105415 (1978-08-01), Lovett
patent: 4299916 (1981-11-01), Litman et al.
patent: 4540659 (1985-09-01), Litman et al.
patent: 4596768 (1986-06-01), Singh et al.
Bleile Dennis M.
Khanna Pyare
Stiso Cynthia D.
Leitereg Theodore J.
Syntex (U.S.A.) Inc.
Wagner Richard
Warden Robert J.
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