Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Reexamination Certificate
2006-07-21
2011-12-27
Noakes, Suzanne M (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
C530S345000
Reexamination Certificate
active
08084226
ABSTRACT:
A method for site-specific modification of protein molecules includes providing a protein molecule having at least first and second non-terminal Cys residues at respective first and second sites within the protein molecule, providing a blocking molecule that is suitable to interact with the protein molecule to selectively block the second non-terminal Cys residue to prevent modifications of the second non-terminal Cys residue while leaving the first non-terminal Cys residue unblocked, and providing a Cys-modifying molecule that is suitable to modify the first non-terminal Cys residue. The blocking molecule according to an embodiment of the invention may be a protein molecule, DNA, an aptamer or synthetic organic molecules. The Cys-modifying molecule according to an embodiment of the invention may be a fluorophore, biotin, spin label, a sugar or a non-natural amino acid analogue.
REFERENCES:
Jager et al., Protein-protein interactions as a tool for site-specific labeling proteins, Protein Science, published online: Jun. 29, 2005, 14: 2059-2068.
Ngo et al., in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Onuchic, J. N.; Luthey-Schulten, Z.; Wolynes, P. G.Ann. Rev. Phys. Chem. 1997, 48, 545-600.
Dill, K. A.; Chan, H. S.Nat. Struct. Biol. 1997, 4, 10-19.
Shea, J. E.; Brooks 3rd, C. L.Ann. Rev. Phys. Chem. 2001, 52, 499-535.
Talaga, D. S.; Lau, W. L.; Roder, H.; Tang, J.; Jia, Y.; DeGrado, W. F.; Hochstrasser, R. M.Proc. Natl. Acad. Sci USA2000, 97, 13021-13026.
Deniz, A. A.; Laurence, T. A.; Beligere, G. S.; Dahan, M.; Martin, A. B.; Chemla, D. S.; Dawson, P. E.; Schultz, P. G.; Weiss, S.Proc. Natl. Acad. Sci USA2000, 97, 5197-5184.
Schuler, B.; Lipman, E. A.; Eaton, W. A.Nature2002, 419, 743-747.
Rhoades, E.; Gussakovsky, E.; Haran, G.Proc. Natl. Acad. Sci USA2003, 100, 3197-3202.
Rhoades, E.; Cohen, M.; Schuler, B.; Haran, G.J. Am. Chem. Soc. 2004.
Lipman, E. A.; Schuler, B.; Bakajin, O.; Eaton, W. A.Science2003, 301, 1233-1235.
Weiss, S.Nat. Struct. Biol. 2000, 7, 724-729.
Selvin, P. R.Nat. Struct. Biol. 2000, 7, 730-734.
Deniz, A. A.; Laurence, T. A.; Dahan, M.; Chemla, D. S.; Schultz, P. G.; Weiss, S.Annu. Rev. Phys. Chem. 2001, 52, 233-253.
Ha, T.Methods2001, 25, 78-86.
Ha, T.Biochemistry2004, 43, 4055-4063.
Clegg, R. M.Methods Enzymol. 1992, 211, 353-388.
Stryer, L.; Haugland, R. P.Proc. Natl. Acad. Sci. USA1967, 58, 719-726.
Jia, Y.; Talaga, D. S.; Lau, W. L.; Lu, H. S. M.; DeGrado, W. F.; Hochstrasser, R. M.Chem. Phys. 1999, 247, 69-83.
Hohng, S.; Joo, C.; Ha, T.Biophys. J. 2004, 87, 1328-1337.
Liu, J.; Lu, Y.J. Am. Chem. Soc. 2002, 124, 15208-15216.
Watrob, H. M.; Pan, C.; Barkley, M. D.J. Am. Chem. Soc. 2003, 125, 7336-7343.
Sinev, M.; Landsmann, P.; Sineva, E.; Ittah, V.; Haas, E.Bioconjugate Chem. 2000, 11, 352-362.
Ratner, V.; Kahana, E.; Eichler, M.; Haas, E.Bioconjugate Chem. 2002, 13, 1163-1170.
Moerner, W. E.; Orrit, M.Science1999, 283, 1670-1676.
Brasselet, S.; Moerner, W. E.Single Molecules2000, 1, 17-23.
Dawson, P. E.; Muir, T. W.; Clark-Lewis, I.; Kent, S. B.Science1994, 266, 776-779.
Muir, T. W.; Sondhi, D.; Cole, P. A.Proc. Natl. Acad. Sci. USA1998, 95, 6705-6710.
Tolbert, T. J.; Wong, C. H.Angew. Chem. Int. Ed. 2002, 41, 2171-2174.
Schuler, B.; Pannell, L. K.Bioconjugate Chem. 2002, 13, 1039-1043.
Geoghegan, K. F.; Stroh, J. G.Bioconjugate Chem. 1992, 3, 138-146.
Shao, J.; Tam, J. P.J. Am. Chem. Soc. 1995, 117, 3893-3898.
Zhang, L.; Tam, J. P.Anal. Biochem. 1996, 233, 87-93.
Guillaumie, F.; Thomas, O. R. T.; Jensen, K. J.Bioconjugate Chem. 2002, 13, 285-294.
Chelius, D.; Shaler, T. A.Bioconjugate Chem. 2003, 14, 205-211.
Chin, J. W.; Santoro, S. W.; Martin, A. B.; King, D. S.; Wang, L.; Schultz, P. G.J. Am. Chem. Soc. 2002, 124, 9026-9027.
Deiters, A.; Cropp, T. A.; Mukherji, M.; Chin, J. W.; Anderson, J. C.; Schultz, P. G.J. Am. Chem. Soc. 2003, 125, 11782-11783.
Wang, L.; Zhang, Z.; Brock, A.; Schultz, P. G.Proc. Natl. Acad. Sci USA2002, 100, 56-61.
Zhang, Z.; Smith, B. A. C.; Wang, L.; Brock, A.; Cho, C.; Schultz, P. G.Biochemistry2003.
Janin, J.Proteins1997, 28, 153-161.
Lo Conte, L.; Chothia, C.; Janin, J.J. Mol. Biol. 1999, 285, 2177-2198.
Jones, S.; Marin, A.; Thornton, J. M.Protein Eng. 2000, 13, 77-82.
Chakrabarti, P.; Janin, J.Proteins2002, 47, 334-343.
Radisky, E. S.; Koshland Jr., D. E.Proc. Natl. Acad. Sci USA2002, 99, 10316-10321.
Radisky, E. S.; Koshland Jr., D. E.Biochemistry2003, 42, 6484-6492.
Bryan, P.; Wang, I.; Hoskins, J.; Ruvinov, S.; Strausberg, S.; Alexander, P.; Almog, O.; Gilliland, G.; Gallagher, T.Biochemistry1995, 34, 10310-10318.
Pace, C. N.Methods Enzymol. 1986, 131, 266-280.
Santoro, M. M.; Bolen, D. W.Biochemistry1988, 27, 8063-8068.
Myers, J. K.; Pace, C. N.; Scholtz, J. M.Protein Sci. 1995, 4, 2138-2148.
Kapanidis, A. N.; Lee, N. K.; Laurence, T. A.; Doose, S.; Margeat, E.; Weiss, S.Proc. Natl. Acad. Sci USA2004, 101, 8936-8941.
Dahan, M.; Deniz, A. A.; Ha, T.; Chemla, D. S.; Schultz, P. G.; Weiss, S.Chem. Phys. 1999, 247, 85-106.
Bogan, A. A.; Thom, K. S.J. Mol. Biol. 1998, 280, 1-9.
Jackson, S. E.; Fersht, A. R.Biochemistry1991, 30, 10428-10435.
Jackson, S. E.; Fersht, A. R.Biochemistry1994, 33, 13880-13887.
Radisky, E. S.; Kwan, G.; Lu, K.; Koshland Jr., D. E.Biochemistry2004, 43, 13648-13656.
Eftink, M.Methods Enzymol. 1997, 278, 221-257.
Deniz, A. A.; Dahan, M.; Grunwell, J. R.; Ha, T.; Faulhaber, A. E.; Chemla, D. S.; Weiss, S.; Schultz, P. G.Proc. Natl. Acad. Sci. USA1999, 96, 3670-3675.
Buckle, A. M.; Schreiber, G.; Fersht, A. R.Biochemistry1994, 33, 8878-8889.
Welch, M.; Chinardet, N.; Mourey, L.; Birck, C.; Samama, J. P.Nat. Struct. Biol. 1998, 5, 25-29.
Lim, D.; Park, H. U.; De Castro, L.; Kang, S. G.; Lee, H. S.; Jensen, S.; Lee, K. L.; Strynadka, N. C. J.Nat. Struct. Biol. 2001, 8, 848-852.
Fieulaine, S.; Morera, S.; Poncet, S.; Mijakovic, I.; Galinier, A.; Janin, J.; Deutscher, J.; Nessler, S.Proc. Natl. Acad. Sci USA2002, 99, 13437-13441.
Kelley, R. F.; O'Connell, M. P.Biochemistry1993, 32, 6828-6835.
Hawkins, R. E.; Russell, S. J.; Baier, M.; Winter, G.J. Mol. Biol. 1993, 234, 958-964.
Li, H.; Dunn, J. J.; Luft, B. J.; Lawson, C. L.Proc. Natl. Acad. Sci USA1997, 94, 3584-3589.
Ding, W.; Huang, X.; Yang, X.; Dunn, J. J.; Luft, B. J.; Koide, S.; Lawson, C. L.J. Mol. Biol. 2000, 302, 1153-1164.
Mandell, J. G.; Falick, A. M.; Komives, E. A.Proc. Natl. Acad. Sci USA1998, 95, 14705-14710.
Hughes, C. A.; Mandell, J. G.; Anand, G. S.; Stock, A. M.; Komives, E. A.J. Mol. Biol. 2001, 307, 967-976.
Anand, G. S.; Law, D.; Mandell, J. G.; Snead, A. S.; Tsigelny, I.; Taylor, S. S.; Ten Eyck, L. F.; Komives, E. A.Proc. Natl. Acad. Sci. USA2003, 100, 13264-13269.
Chu, F.; Shan, S.; Moustakas, D. T.; Alber, F.; Egea, P. F.; Stroud, R. M.; Walter, P.; Burlingame, A. A.Proc. Natl. Acad. Sci USA2004, 101, 16454-16459.
Feldhaus, M. J.; Siegel, R. W.; Opresko, L. K.; Coleman, J. R.; Feldhaus, J. M.; Yeung, Y. A.; Cochran, J. R.; Heinzelman, P.; Colby, D.; Swers, J.; Graff, C.; Wiley, H. S.; Wittrup, K. D.Nat. Biotechnol. 2003, 21, 163-170.
Jäger Marcus
Weiss Shimon
Axelrod Nancy J.
Daley Henry J.
Lee Jae W
Noakes Suzanne M
The Regents of the University of California
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