Determination of a chlamydial or gonococcal antigen using a posi

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 28, 436518, 436531, 436540, C12Q 100, C12Q 128, G01N 3353

Patent

active

050752200

ABSTRACT:
Antigens extracted from chlamydial or gonococcal organism are rapidly and ionically bound directly to a polymeric solid support which has cationic groups on its surface. The support is substantially free of any antibody or other biological compound reactive with the antigen. Ionically bound antigen is then contacted with suitable chlamydial or gonococcal antibodies to form a bound immunological complex which is detected in suitable fashion, for example by using a labeled anti-body. The entire assay, carried out at room temperature, requires less than 30 minutes and is highly sensitive.

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