Methods of chromosome drying and spreading

Details Methods of chromosome drying and spreading Methods of chromosome drying and spreading

2006-10-11

2011-11-15

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

08057995

ABSTRACT:
The present invention provides for a method of drying and spreading chromosomes from various biological samples to yield optimal chromosomal spreading. The method requires preparing a biological sample for treatment, providing a cytogenetic chamber capable of setting predetermined conditions, pre-testing a portion of the biological sample in the cytogenetic chamber, and finally treating the remaining biological sample. The method is useful to yield metaphase chromosomes that are small and rounded, with very few overlapping or scattered chromosomes. Furthermore, the method is uses restricted ranges of temperature and relative humidity to achieve consistent chromosomal spreading. The morphologies of the chromosomes are preserved in order to execute banding techniques at 550 bands and chromosomal analysis on high-resolution chromosomes.

REFERENCES:
patent: 5851790 (1998-12-01), Walker et al.
patent: 2001/0016351 (2001-08-01), Sorge et al.
patent: 2001/0046682 (2001-11-01), Lichtenwalter
patent: 2005/0042767 (2005-02-01), Machida et al.
patent: 2006/0154263 (2006-07-01), Vallabhaneni
Preist J.H. Cytogenetics Chapter 4-7 pp. 52-92 Editors French et al. published by Lea & Febiger Philadelphia, PA (1969).
Boyle et al. Chapter 3 : “The Cytogenetics of pregnancy” in “Human Cytogenetics :constitutional analysis” 3rd Edition; Editor Rooney, D. E.; published by Oxford University Press ; New York, NY (2001).
Henegariu et al., Improvements in cytogenetic slide preparation : Controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry 43 : 101-109 (2001).
Zvarich MT, et al., “Automation in the Cytogenetic Laboratory Offers Significant Benefits,”Association of Cytogenetic Technologists, Fifteenth Annual Meeting, Santa Fe, New Mexico, 1990.
Zvarich, Michael, et al., “Robotic Harvest of Phytohaemagglutinin—Stimulated Peripheral Blood Lymphocyte Cultures,”Association of Cytogenetic Technologists, Fifteenth Annual Meeting, Sante Fe, New Mexico, 1990.
Buchanan PB, Lab Management and Automated Karyotyping: Seventh Annual Meeting of the Southeastern Regional Genetics Group, Atlanta, GA (1987).
Buchanan PD, Laundon CH: Early Amniocentesis and Computer Assisted Cytogenetics, Seventh Annual Meeting of the Southeastern Regional Genetics Group, Atlanta GA (Mar. 1989).
Buchanan PD, Laundon CH: Lab Management and Automation, Eighth Annual Meeting of the Southeastern Regional Genetics Group, Atlanta GA (1990).
Zvarich MT, et al., “Robotic Harvest of Phytohaemagglutinin—Stimulated Peripheral Blood Lymphocyte Cultures,” Southern Genetics Group, Thirteenth Summer Meeting, San Destin, FL (1990).
Zvarich MT, et al., “Robotic Harvest of Blood Cultures,” Society of Perinatal Obstetricians, Orlando, Florida (1992).

Affiliated with

Also associated with

Rating

Methods of chromosome drying and spreading does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of chromosome drying and spreading, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of chromosome drying and spreading will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4292608