Transgenic plants with reduced level of saturated fatty acid...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S281000, C435S320100, C435S419000, C536S023100

Reexamination Certificate

active

08063269

ABSTRACT:
The present invention provides transgenic plants with reduced levels of saturated fatty acids in the seed oil and methods of making these plants. The transgenic plants developed through this method contain reduced levels of saturated fatty acids in seed oil due to expression of a prokaryotic delta-9 desaturase enzyme (i.e. an enzyme that introduces cis double bonds at the delta-9 position of saturated fatty acids) operably linked with an endoplasmic reticulum retention and retrieval signal sequence. One example of the invention is a plant expressing a heterologous delta-9 desaturase enzyme from cyanobacteriumAnacystis nidulans, which converts lipid-bound 16:0 and 18:0 fatty acids into corresponding 16:1 and 18:1, in operative linkage with a KKSS (SEQ ID NO:5) endoplasmic reticulum retention and retrieval signal sequence.

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