Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2008-09-26
2011-11-01
Kosson, Rosanne (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S194000, C435S234000, C435S252300
Reexamination Certificate
active
08048649
ABSTRACT:
Methods and compositions for increased production of amino acids fromC. glutamicumusing sucrose as a carbon source are described. In one aspect, increased production of L-lysine fromC. glutamicumis accomplished by using a strain having a mutation in the ptsF gene encoding fructose-PTS enzyme that attenuates or blocks fructose import into the cell when such strain is grown on media containing sucrose as a carbon source and production is increased by providing glucose isomerase in the fermentation media. The glucose isomerase may be exogenously added or expressed in the strain and exported into the media. In certain embodiments the media also contain an invertase. In another aspect increased production of L-lysine is accomplished by making aC. glutamicumstrain having the ptsF mutation and a second mutation in a fructose exporter function. The dual mutation retains imported fructose in the cell. In certain embodiments, the strain also overexpresses at least one of a glucose isomerase and glucokinase activity in the cell to drive imported fructose toward the pentose phosphate pathway to increase L-lysine production.
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Binder Thomas P.
Hanke Paul D.
Archer Daniels Midland Company
Kosson Rosanne
Roberts Mark W.
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