Melting curve analysis with exponential background subtraction

Details Melting curve analysis with exponential background subtraction Melting curve analysis with exponential background subtraction

2006-09-20

2011-11-29

Brusca, John S (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C700S001000, C435S006120

Reexamination Certificate

active

08068992

ABSTRACT:
A system and methods are provided for melting curve genotyping analysis of nucleic acids. Melting curves are generated by plotting fluorescence of a sample as a function of temperature. In one illustrative example, an exponential algorithm is employed to remove the background from generated melting curves and thereby perform comparative analysis to other melting curves. Additional illustrative examples provide for measuring the differences between two or more melting curves and clustering the genotypes of the provided sample nucleic acids.

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