Microfluidic control chip and method of detecting protein...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S325000, C436S518000, C436S520000, C436S532000, C436S546000, C427S002130

Reexamination Certificate

active

08053201

ABSTRACT:
Provided is a microfluidic control chip, which includes a filter section having a filter to which anti-immunoglobulin antibodies, which are bound to endogenous antibodies in blood to thereby remove the endogenous antibodies, are immobilized, a first reaction section to which detection antibodies immobilized to fluorescent nano-particles are adsorbed, the detection antibodies being bound to proteins to be detected in blood which is introduced from the filter section with the endogenous antibodies removed therefrom, and a second reaction and detection section including capture antibodies immobilized thereto, binding the capture antibodies to the proteins, which are bound to the detection antibodies introduced from the first reaction section, and detecting a concentration of the proteins based on an intensity of fluorescent light. Thus, the microfluidic control chip can minimize interference of an immune response to maximize the immune response.

REFERENCES:
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patent: 2010/0261286 (2010-10-01), Kim et al.
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patent: 1020090006607 (2009-01-01), None
patent: 1020090064942 (2009-06-01), None
Zhang et al., Biocompatibility evaluation of ePTFE membrane modified with PEG in atmospheric pressure glow discharge, 2002, J Biomedical Materials Research, 60(3): pp. 502-509.
Susann Eriksson et al., “Negative Interference in Cardiac Troponin I Immunoassays by Circulating Troponin Autoantibodies”, Proteomics and Protein Markers, 2005, pp. 839-847, vol. 51 No. 5, American Association for Clinical Chemistry.

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