Method for forming artificial lipid membrane

Details Method for forming artificial lipid membrane Method for forming artificial lipid membrane

2010-12-17

2011-11-22

Noguerola, Alex (Department: 1759)

Chemistry: electrical and wave energy

Apparatus

Electrolytic

C204S403080, C436S071000, C422S503000

Reexamination Certificate

active

08062489

ABSTRACT:
An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution. The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 μl. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; adding a lipid solution to the artificial lipid membrane forming portion; adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; and forming the artificial lipid membrane.

REFERENCES:
patent: 7408359 (2008-08-01), Ide
patent: 7828947 (2010-11-01), Oki et al.
patent: 2007/0035308 (2007-02-01), Ide
patent: 2007/0161101 (2007-07-01), Takeuchi
patent: 04-215052 (1992-08-01), None
patent: 05-007770 (1993-01-01), None
patent: 07-241512 (1995-09-01), None
patent: 08-152423 (1996-06-01), None
patent: 11-056389 (1999-03-01), None
patent: 2005-91308 (2005-04-01), None
patent: 2005-098718 (2005-04-01), None
patent: 2006-312141 (2006-11-01), None
patent: 2007-187560 (2007-07-01), None
patent: 2008-194573 (2008-08-01), None
patent: 2009-008618 (2009-01-01), None
patent: WO 2005/071405 (2005-08-01), None
Kestin et al. The Viscosity of Aqueous KCI Solutions in the Temperature Range 25-200°C. and the Pressure Range 0.1-30 MPa, International Journal for Thermophysics, vol. 2, No. 4, 1981.
Yasunobu Okada, “Patch Clamp Experimental Technique,” Yoshioka Book Store, Sep. 25, 1996, pp. 133-139.

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