Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-05-18
2011-12-13
Carlson, Karen (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C536S023100
Reexamination Certificate
active
08076102
ABSTRACT:
The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells utilizing any enzyme useful in the eukaryotic sterol/cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker to select transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in medium lacking cholesterol and/or producing the heterologous protein in these cells in chemically defined and/or serum-free media.
REFERENCES:
patent: 5110737 (1992-05-01), Myoken et al.
patent: WO 2006099369 (2006-09-01), None
Abbaszade et al. 1995; The mouse 3—-hydroxysteroid dehydrogenase multigene family includes two functionally distinct groups of proteins. Molecular Endocrinology 9: 1214-1222.
Nokelainen et al. 1998; Expression cloning of a novel estrogenic mouse 17—-hydroxysteroid deyhydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRPA) in rat. Molecular Endocrinology. 12: 1048-1059.
Abbaszade et al., “The Mouse 3 beta-Hydroxysteroid Dehydrogenase Multigene Family Includes Two Functionally Distinct Groups of Proteins,” Molecular Endocrinology, Sep. 1995, vol. 9, pp. 1214-1222, pp. 1215 and 1216, Fig. 1, 4.
Nokelainen et al., “Expression Cloning of a Novel Estrogenic Mouse 17 beta-Hydroxysteroid Dehydrogenase/17-Ketosteroid Reductase (m17HSD7), Previously Described as a Prolactin Receptor-Associated Protein (PRAP) in Rat,” Molecular Endocrinology, Jul. 1998, vol. 12, pp. 1048-1059, p. 1051, Fig. 2.
Zhou et al., “Fed-Batch Culture Recombinant NS0 Myeloma Cells with High Monoclonal Antibody Production,” Biotechnol Bioeng. Sep. 1997, vol. 55(5), pp. 783-792, p. 784 (Abstract).
G. Seth et al., “17β-Hydroxysteroid Dehydrogenase Type 7 (Hsd17b7) Reverts Cholesterol Auxotrophy in NS0 Cells,” Journal of Biotechnology, 2006, vol. 121, pp. 241-252.
Gen Bank: Accession No. L41519, Mus Musculus 3-Ketosteroid Reductase (HSD3b5) mRNA, 1997.
Gen Bank: Accession No. BC011464, Mus Musculus Hydroxysteroid (17-beta) Dehydrogenase 7, mRNA (cDNA Clone MGC:11432 Image:3966186), 2005.
I. Dufort et al., “Characteristics of a Highly Labile Human Type 5 17β-Hydroxysteroid Dehydrogenase,” Endocrinology, 1999, vol. 140, No. 2, pp. 568-574.
Z. Marijanovic et al., “Closing the Gap: Identification of Human 3-Ketosteroid Reductase, the Last Unknown Enzyme of Mammalian Cholesterol Biosynthesis,” Molecular Endocrinology, Sep. 2003, vol. 17, No. 9, pp. 1715-1725.
Jayme DW, Smith SR (2000). “Media formulation options and manufacturing process controls to safeguard against introduction of animal origin contaminants in animal cell culture.”.Cytotechnology33: 27-36. (Abstract).
Branco Luis
Sampey Darryl
Biofactura, Inc.
Carlson Karen
Womble Carlyle Sandridge & Rice PLLC
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