Nucleic acid-based detection

Details Nucleic acid-based detection Nucleic acid-based detection

2006-12-22

2011-12-06

Chong, Kimberly (Department: 1635)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C436S501000, C435S006120, C435S018000, C435S019000

Reexamination Certificate

active

08071734

ABSTRACT:
The invention relates to compositions, systems, and methods for simultaneously detecting the presence and quantity of one or more different compounds in a sample using aptamer beacons. Aptamer beacons are oligonucleotides that have a binding region that can bind to a non-nucleotide target molecule, such as a protein, a steroid, or an inorganic molecule. New aptamer beacons having binding regions configured to bind to different target molecules can be used in solution-based and solid, array-based systems. The aptamer beacons can be attached to solid supports, e.g., at different predetermined points in two-dimensional arrays. The invention includes devices, methods, and computer software for carrying out the methods.

REFERENCES:
patent: 4781458 (1988-11-01), Angel et al.
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5270163 (1993-12-01), Gold et al.
patent: 5485277 (1996-01-01), Foster
patent: 5567588 (1996-10-01), Gold et al.
patent: 5593835 (1997-01-01), Rando et al.
patent: 5674688 (1997-10-01), Kauvar et al.
patent: 5691145 (1997-11-01), Pitner et al.
patent: 5771097 (1998-06-01), Kusunose et al.
patent: 5837466 (1998-11-01), Lane et al.
patent: 5843653 (1998-12-01), Gold et al.
patent: 5874219 (1999-02-01), Rava et al.
patent: 5989823 (1999-11-01), Jayasena et al.
patent: 6037130 (2000-03-01), Tyagi et al.
patent: 6103476 (2000-08-01), Tyagi et al.
patent: 6147204 (2000-11-01), Gold et al.
patent: 6150097 (2000-11-01), Tyagi et al.
patent: 0826780 (1998-03-01), None
patent: 10-293128 (1998-11-01), None
patent: WO97/38134 (1997-10-01), None
patent: WO 97/42345 (1997-11-01), None
patent: WO 97/49989 (1997-12-01), None
patent: WO98/10096 (1998-03-01), None
patent: WO/99/05314 (1999-02-01), None
patent: WO99/31275 (1999-06-01), None
patent: WO00/06778 (2000-02-01), None
Bacher et al., “Nucleic Acid Selection as a Tool for Drug Discovery,”Drug Discovery Today, 3(6):265-273 (1998).
Cardullo et al., “Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer,”Proc. Natl. Acad. Sci. USA, 8:8790-8794 (Dec. 1988).
Ciesiolka et al., “Affinity Selection-Amplification from Randomized Ribooligonucleotide Pools,”Methods in Enzymology, 267:315-35 (1996).
Conrad et al., “Detecting Immobilized Protein Kinase C Isozymes with RNA Aptamers,”Analytical Biochemistry, Article No. 0462, 242:261-265 (1996).
Conrad et al., “In Vitro Selection of Nucleic Acid Aptamers That Bind Proteins,”Methods in Enzymology, 267:336-383 (1996).
Davis et al., “Staining of cell surface human CD4 with 2′-F-pyrimidine-containing RNA aptamers for flow cytometry,”Nucleic Acids Research, 26(17):3915-3924 (1998).
Ellington et al., “In vitro selection of RNA molecules that bind specific ligands,”Nature, 346:818-822 (1990).
Fang et al., “Designing a Novel Molecular Beacon for Surface-Immobilized DNA Hybridization Studies”,Journal of the Am. Chem Society, 121(12):2921-2922 (1999).
Feigon et al., “Aptamer Structures From A to Zeta”,Chemistry&Biology, 3(8):611-617 (1996).
Fitzwater et al., “A SELEX Primer,”Methods in Enzymology, 267:275-301 (1996).
Gold et al., “Diversity of Oligonucleotide Functions,”Annu. Rev. Biochem., 64:763-97 (1995).
Gold, “Oligonucleotides as Research, Diagnostic, and Therapeutic Agents,”The American Society for Biochemistry and Molecular Biology, Inc., 270(23):13581-13584 (1995).
Griffiths et al.,The EMBO Journal, 13:3245-3260 (1994).
Hamaguchi et al., “Aptamer Beacons for the Direct Detection of Proteins”,Analytical Biochemistry, 294(2):126-131 (2001).
Jayasena, “Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics.”Clin. Chem., 45(9):1628-50 (1999).
Kramer, “Molecular Beacons: Hybridization Probes for Detection of Nucleic Acids in Homogenous Solutions,” http://www.molecular-beacons.com (Dec. 7, 1998).
Lashkari et al., “Yeast Microarrays for Genome Wide Parallel Genetic and Gene Expression Analysis,”Proc. Nat'l Acad. Sci. USA, 94:13057-62 (1997).
Lin et al., “Modified RNA sequence pools for in vitro selection,”Nucleic Acids Research, 22(24):5229-5234 (1994).
Morgan et al., “A surface plasmon resonance immunosensor based on the streptavidin-biotin complex,”Biosensors&Bioelectronics, 7:405-410 (1992).
Morris et al., “High affinity ligands from in vitro selection: complex targets.”Proc. Natl. Acad. Sci., USA, 95(6):2902-7 (1998).
Osborne et al., “Aptamers as therapeutic and diagnostic reagents: problems and prospects,”Chemical Biology, 1:5-9 (1997).
Pagratis et al., “Potent 2′-amino-, and 2′-fluoro-2′-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor,”Nature Biotechnology,15:68-73 (1997).
Patel et al., “Structure, Recognition and Adaptive Binding in RNA Aptamer Complexes”,Jour. Of Molecular Biology, 272(5):645-664 (1997).
Pease et al., “Light Generated Oligonucleotide Arrays for Rapid DNA Sequence Analysis,”Proc. Nat'l Acad. Sci. USA, 91:5022-26 (1994).
Potyrailo et al., “Adapting Selected Nucleic Acid Ligands (Aptamers) to Biosensors,”Anal. Chem., 70:3419-3425 (1998).
Shalon et al., “A DNA Microarray System for Analyzing Complex DNA Samples Using Two-Color Fluorescent Probe Hybridization,”Genome Res., 6:639-45 (1996).
Schena et al., “Parallel Human Genome Analysis: Microarray-Based Expression Monitoring of 1000 Genes,”Proc. Nat'l Acad. Sci. USA, 93: 10514-19 (1996).
Sixou et al., “Intracellular Oligonucleotide Hybridization Detected by Fluorescence Resonance Energy Transfer (FRET),”Nucleic Acids Res., 22:662-68 (1994).
Tasset et al., “Oligonucleotide Inhibitors of Human Thrombin that Bind Distinct Epitopes”,Journal of Molecular Biology, 272(5):688-698 (1997).
Tyagi et al., “Molecular Beacons: Probes That Fluoresce Upon Hybridization,”Nature Biotech., 14:303-08 (1996).

Affiliated with

Also associated with

Rating

Nucleic acid-based detection does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid-based detection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid-based detection will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4271271