Method of detecting Streptococcus pneumoniae, primer set...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S024000

Reexamination Certificate

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08043812

ABSTRACT:
A method of detectingStreptococcus pneumoniae, which is characterized in that it comprises amplifying a lytA gene derived fromS. pneumoniaeusing a LAMP primer set comprising at least one primer having a nucleotide sequence that is identical to or complementary to a partial sequence in the region ranging from bp 40 to 450 of the nucleotide sequence region of the above-described lytA gene, and then detecting the obtained amplified product.

REFERENCES:
patent: 2003/0207292 (2003-11-01), Notomi et al.
Seki, M. et al. Loop-mediated amplification method targeting the lytA gene for setection ofStreptococcus pneumoniae.J Clin Microbiol., vol. 43, No. 4, pp. 1581-1586, Apr. 2005.
Lowe et al., A computer program for selection of oligonucleotide primers for polymerase chain reactions. Nucleic Acids Research, vol. 18, No. 7, pp. 1757-1761, 1990.
Whatmore, A.M. et al. The autolysin-encoding gene (lytA) ofStreptococcus pneumoniaedisplays restricted alleleic variation despite localized recombination events with genes of pneumococcal bacteriophage encoding cell wall lytic enzymes. Infection and Immunity, vol. 67, No. 9, pp. 4551-4556, 1999.
Seki, M. et al., J. Clin. Microbial., (Apr. 2005), vol. 43, No. 4, pp. 1581 to 1586.
Sheppard, C.L. et al., J. Medical Microbiology, (Mar. 2004), vol. 53, pp. 189 to 195.
S. pneumoniaeautolysin (lytA) gene, complete cds, GenBank [Online], Apr. 26, 1993, Accession No. M13812 Retrieved on Nov. 22, 2005, Retrieved from the United States NCBI web site.
Nagamine, K. et al., Mol. Cell. Probes, (2002), vol. 16, pp. 223 to 229.
Gillespie, S.H. et al., J. Clin. Microbial., (1994), vol. 32, No. 5, pp. 1308 to 1311.
Rudolph, K.M. et al., J. Clin. Microbial., (1993), vol. 31, No. 10, pp. 2661 to 2666.

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