Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Reexamination Certificate
1998-04-10
2010-06-29
McElwain, Elizabeth F (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
C800S298000
Reexamination Certificate
active
07745694
ABSTRACT:
The present invention relates to compositions and methods for preparing polyunsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding fatty acid desaturases, including Δ5-desaturases, Δ6-desaturases and Δ12-desaturases, are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more desaturases. Expression of the desaturases with different substrate specificities in the plant system permit the large scale production of polyunsaturated long chain fatty acids such as docosahexaenoic acid, eicosapentaenoic acid, α-linolenic acid, gamma-linolenic acid, arachidonic acid and the like for modification of the fatty acid profile of plants, plant parts and tissues. Manipulation of the fatty acid profiles allows for the production of commercial quantities of novel plant oils and products.
REFERENCES:
patent: 3649295 (1972-03-01), Bernhart
patent: 4058594 (1977-11-01), Williams
patent: 4526793 (1985-07-01), Ingenbleek et al.
patent: 4526902 (1985-07-01), Rubin
patent: 4614663 (1986-09-01), Rule
patent: 4670285 (1987-06-01), Clandinin et al.
patent: 4843095 (1989-06-01), Rubin
patent: 4920098 (1990-04-01), Cotter et al.
patent: 4938984 (1990-07-01), Traitler et al.
patent: 5057419 (1991-10-01), Martin
patent: 5374657 (1994-12-01), Kyle
patent: 5376541 (1994-12-01), Kawashima
patent: 5407957 (1995-04-01), Kyle et al.
patent: 5443974 (1995-08-01), Hilz
patent: 5492938 (1996-02-01), Kyle et al.
patent: 5512482 (1996-04-01), Voelker et al.
patent: 5545553 (1996-08-01), Gotschlich
patent: 5550156 (1996-08-01), Kyle
patent: 5552306 (1996-09-01), Thomas
patent: 5614393 (1997-03-01), Thomas
patent: 5614400 (1997-03-01), Cahoon
patent: 5663068 (1997-09-01), Thomas
patent: 5689050 (1997-11-01), Thomas
patent: 5789220 (1998-08-01), Thomas
patent: 5968809 (1999-10-01), Knutzon et al.
patent: 5972664 (1999-10-01), Knutzon et al.
patent: 6051754 (2000-04-01), Knutzon et al.
patent: 6075183 (2000-06-01), Knutzon et al.
patent: 6136574 (2000-10-01), Knutzon et al.
patent: 6355861 (2002-03-01), Thomas
patent: 6459018 (2002-10-01), Knutzon
patent: 6683232 (2004-01-01), Thomas
patent: 7189894 (2007-03-01), Thomas
patent: 550162 (1993-07-01), None
patent: 0561569 (1993-09-01), None
patent: 644263 (1995-03-01), None
patent: 736598 (1996-01-01), None
patent: WO 91/13972 (1991-09-01), None
patent: WO 93/06712 (1993-04-01), None
patent: WO 93/11245 (1993-06-01), None
patent: WO 94/11516 (1994-05-01), None
patent: WO 94/18337 (1994-08-01), None
patent: WO 96/10086 (1996-04-01), None
patent: WO 96/13591 (1996-05-01), None
patent: WO 96/21022 (1996-07-01), None
patent: WO 96/21037 (1996-07-01), None
patent: WO 97/30582 (1997-08-01), None
Ackman, “Problems in Fish Oils and Concentrates,” Canadian Institute of Fisheries Technology, Technical University of Nova Scotia, 189-204.
Bajpai and Bajpai, “Arachidonic Acid Production by Microorganisms,” Biotechnology and Applied Biochemistry 15:1-10 (1992).
Gurr, “Alpha or Gamma: What's a Double Bond Position Between Friends?” 1. Gamma-linolenic Acid, Lipid Technology (Mar. 1995).
Hodgson, “Advances in Vector Systems for Gene Therapy,” Ex. Opin. Ther. Patents 5(5):459-468 (1995).
Horrobin, “Medical Roles of Metabolites of Precursor EFA,” INFORM 6(4):428-434 (Apr. 1995).
Murata at al., “Biosynthesis of gamma-Linolenic Acid in the Cyanobacterium Spiruline platensis,” In: gamma-Linolenic Acid Metabolism and Its Roles in Nutrition and Medicine (Huang and Mills, eds.), pp. 22-32, Access Press , Champain, IL.
Ratledge, “Single cell oils-have they a biotechnological future?” MB Tech. 11 (Jul. 1995).
Reddy and Thomas, “Expression of a cyanobacterial Δ6-desaturase gene results in gamma-linolenic Acid Production in Transgenic Plants,” Nature Biotechnology 14:639-642 (May 1996).
Ward, “Microbial Production of long-chain PUFAs,” INFORM 6(6):683-688 (Jun. 1995).
“Closer to Mother's Milk”, the Gist 61:8-9 (Spring 1995).
“Exciting Prospects for Stearidonic Acid Seed Oils,” Lipid Technology (Nov. 1996).
Takagi and Itabashi, “cis-5-Olefinic Unusual Fatty Acids in Seed Lipids of Gymnospermae and Their Distribution in Triacygylycerols, ” Lipids 17(10):716-723(1982).
Wolff, “New Tools to Explore Lipid Metabolism,” INFORM 8(1):116-119 (Jan. 1997).
“Conifer Oils Offer Exciting Possibilities,” Lipid Technology (Jan. 1997).
PCT International Search Report.
L.V. Michaelson, et al., “Isolation of a Δ5-Fatty Acid Desaturase Gene from Mortierella alpina”, Journal of Biological Chemistry, American Society of Biological Chemists, vol. 273, No. 30, Jul. 24, 1998, pp. 19055-19059 (XP-002076636).
Deborah S. Knutzon, et al., “Identification of Δ5-Desaturase from Mortierella alpina by Heterologous Expression in Bakers' Yeast and Canola”, Journal of Biological Chemistry, American Society of Biological Chemists, vol. 273, No. 45, Nov. 6, 1998, pp. 29360-29366 (XP-002106760).
Patrick S. Covello, et al., “Functional Expression of the ExtraplastidialArabidopsis thalianaOleate Desaturase Gene (FAD2) inSaccharamyces cerevisiae”, Plant Physiol., vol. 111, 1996, pp. 223-226 (XP-002075211).
U.S. Appl. No. 09/367,013, filed Apr. 10, 1998, Knutzon, et al.
Hill-Eubanks, et al.; “Structure of a G-Protein-Coupling domain of a Muscarinic Receptor Predicted by Random Saturation Mutagenesis”; The Journal of Biological Chemistry; vol. 271, No. 6, Feb. 9, 1996, pp. 3058-3065; The American Society for Biochemistry and Molecular Biology, Inc.
Shibuya, et al.; “Purification, Characterization, and cDNA Cloning of a Novel a-Galactosidase fromMortierella vinacea”; Biosci. Biotech.Biochem., vol. 61, No. 4, 1997; pp. 592-598.
Sambrook, et al.; “Molecular Cloning—A laboratory Manual, Second Edition”; Cold Spring Harbor Laboratory Press, 1989; pp. 8.46-8.49, 9.47-9.57 and 2.114-2.117.
U.S. Appl. No. 11/445,506 and enclosed prosecution history.
Chaudhary Sunita
Huang Yung-Sheng
Knutzon Deborah
Leonard Amanda Eun-Yeong
Mukerji Pradip
Armstrong Teasdale LLP
McElwain Elizabeth F
Monsanto Technology LLC
LandOfFree
Methods and compositions for synthesis of long chain... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for synthesis of long chain..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for synthesis of long chain... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4240053