Chemistry: analytical and immunological testing – Biological cellular material tested
Reexamination Certificate
2004-10-13
2010-10-12
Le, Long V (Department: 3768)
Chemistry: analytical and immunological testing
Biological cellular material tested
C250S234000, C356S444000, C382S133000, C600S425000, C600S473000
Reexamination Certificate
active
07811825
ABSTRACT:
A scanning method for scanning samples of biological cells using optical tomography includes preparing, acquiring, reconstructing and viewing three-dimensional images of cell samples. Concentration and enrichment of the cell sample follows. The cell sample is stained. Cells are isolated from the cell sample and purified. A cell/solvent mixture is injected into a gel by centrifugation. A cell/gel mixture is injected into a capillary tube until a cell appears centered in a field of view using a stopped-flow method. An optical imaging system, such as a fixed or variable motion optical tomography system acquires a projection image. The sample is rotated about a tube axis to generate additional projections. Once image acquisition is completed, the acquired image projections are corrected for errors. A computer or other equivalent processor is used to compute filtered backprojection information for 3D reconstruction.
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Fauver Mark E.
Nelson Alan C.
Rahn J. Richard
Seibel Eric J.
Citadel Patent Law
Le Long V
Leone George A
University of Washington
VisionGate, Inc.
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