Method for purifying modified major mite allergen

Details Method for purifying modified major mite allergen Method for purifying modified major mite allergen

2004-04-14

2010-02-23

Haddad, Maher M (Department: 1644)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

C530S414000, C530S416000, C530S417000

Reexamination Certificate

active

07667009

ABSTRACT:
A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

REFERENCES:
patent: 5912327 (1999-06-01), Li et al.
patent: 6632425 (2003-10-01), Li et al.
patent: 2002/0054881 (2002-05-01), Sturaro et al.
patent: 2004/0024186 (2004-02-01), Li et al.
patent: 0 819 763 (1998-01-01), None
patent: 6-253851 (1994-09-01), None
patent: 2001-231562 (2001-08-01), None
patent: 2001-231563 (2001-08-01), None
patent: WO 96/30539 (1996-10-01), None
Mukhopadhyay et al. ‘Inclusion bodies and purfication of proteins in biologically active forms.’ Adv. Biochem. Eng. 56:61-109, 1997.
Thomas A.E. Platts-Mills et al; “Dust mites: Immunology, allergic disease, and environmental control”;The Journal of Allergy and Clinical Immunology; vol. 80, pp. 755-775, 1987.
Toshifumi Yuuki et al; “Cloning and Sequencing of cDNAs corresponding to mite major allergen”;Japanese J. Allergology, vol. 39, pp. 557-561, 1990.
“Shin Seikagaku Jikken Koza 1 Tanpakushitsu I-Bunri Seisei Seishitsu” (New Lecture on Biochemical Experiment 1: Isolation, Purification and Property), edited by The Japanese Biochemical Society, published on Feb. 26, 1990, pp. 169-177 and 184-194.
“Tanpakushitsu Kogaku” (Protein Engineering), edited by Kin'ichiro Miura et al, published on Jul. 16, 1988, p. 118.
Toshiro Takai et al; “Engineering of the Major House Dust Mite Allergen DER f 2 for Allergen-Specific Immunotherapy”; Nature Biotechnology; Aug. 15, 1997; pp. 754-758 ISSN: 1087-0156; vol. 15; Publishing Group, New York, NY US.

Affiliated with

Also associated with

Rating

Method for purifying modified major mite allergen does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for purifying modified major mite allergen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for purifying modified major mite allergen will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4232689