Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-10-31
2010-02-16
Robinson, Hope A (Department: 1652)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S422000, C530S350000, C530S300000, C435S007100, C435S325000, C435S252100
Reexamination Certificate
active
07662934
ABSTRACT:
The invention includes a process for extracting a target protein fromE. colicells that includes lowering the pH of a wholeE. colicell solution to form an acidic solution, disrupting the cells to release the protein into the acidic solution, and separating the cellular debris from the released protein to obtain a protein product enriched in the heterologous target protein. The invention also includes addition of a solubility enhancer.
REFERENCES:
patent: 4988798 (1991-01-01), Blum et al.
patent: 5760189 (1998-06-01), Vicik et al.
patent: 6967241 (2005-11-01), Gehant
patent: 2002/0010145 (2002-01-01), Willson, III et al.
patent: 2007/0031930 (2007-02-01), Gehant
patent: 0 229 866 (1987-07-01), None
patent: 1-502556 (1989-09-01), None
patent: 5-503212 (1993-06-01), None
patent: 11-509725 (1999-08-01), None
patent: WO-87/06939 (1987-11-01), None
patent: WO-88/01624 (1988-03-01), None
patent: WO 96/34622 (1996-11-01), None
patent: WO 01/42456 (2001-06-01), None
patent: WO-2004/022581 (2004-03-01), None
Drechsel, D., Geerlof, A., Marco, A.D., Stier, G., Weeks, S., Zandt, H.,The Protein Expression and Purification Flowerchart, Internet Article, ‘Online’ Nov. 10, 2000, XP002357722 EMBL Heidelberg , URL:www.embl.ed/ExternalInfo/protein—unit/draft—frames/Frame—flowchart—ext.htm.
“Expanded Bed Adsorption, Principles and Methods,” Edition AB, pp. 1-160, 1997.
Gehant, R. et al., 2002 (Sep. 8),EBA Conference, Abstract, 1 pg. “Overcoming the Difficulties of Performing CEX-Expanded Bed Chromatography with Low pHE. coliHomogenates”.
Harris, E. et al. (Ed.), “Protein purification methods, a practical approach,” pp. 1-22; Sep. 1, 1990.
Nye, S. et al., 1995,Molecular Immunology, vol. 32, No. 14/15, pp. 1131-1141 “Purification of Immunologically Active Recombinant 21.5 kDa Isoform of Human Myelin Basic Protein”.
Wheelwright, S., “Protein Purification, Design and Scale up of Downstream Processing,” Chapter 5 and 6, 11 pgs., 1991, John Wiley & Sons Inc, New York pp. 52-81.
International Search Report mailed on Feb. 11, 2004, for PCT Application No. PCT/US03/28007, filed on Sep. 5, 2003, two pages.
Supplementary European Search Report mailed on Dec. 23, 2005, for EP Application No. 03752068.1, filed Sep. 5, 2003, three pages.
Genentech Inc.
Morrison & Foerster / LLP
Robinson Hope A
LandOfFree
Process for reducing biomass-biomass or biomass—resin... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for reducing biomass-biomass or biomass—resin..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for reducing biomass-biomass or biomass—resin... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4215245