DNA sequencing method

Details DNA sequencing method DNA sequencing method

1996-12-18

1999-03-02

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

058769340

ABSTRACT:
A method of sequencing a DNA molecule is disclosed. This method comprises the step of exposing the molecule to a mixture of dITP/dGTP in a sequencing elongation reaction whereby compression artifacts are reduced.

REFERENCES:
patent: 5436149 (1995-07-01), Barnes
P.J. Barr, et al., "7-deaza-2'-deoxyguanosine-5'-triphosphate: Enhanced resolution in M13 dideoxy sequencing," BioTechniques 4(5) :428-432, 1986.
M.A. Innis, et al., "DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA," Proc. Natl. Acad. Sci. USA 85:9436-9440, 1988.
D.R. Mills and F.R. Kramer, "Structure-independent nucleotide sequence analysis," Proc. Natl. Acad. Sci. USA 76 (5) :2232-2235, 1979.
S. Tabor and C.C. Richardson, "DNA sequence analysis with a modified bacteriophage T7 DNA polymerase," Proc. Natl. Acad. Sci. USA 84 (14) :7467-4771, 1987.
H. Dierick, et al., "Incorporation of dITP or 7-deaza dGTP during PCR improves sequencing of the product," Nucl. Acids Res. 21 (18) :4427-4428, 1993.
Kambara et al (Biotechnology vol. 9) pp. 648-651, 1991.
Ansorge et al J. Biochem. Biophy. Meth. vol. 20 pp. 47-52, 1989.

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