Total internal reflection holographic microscope

Optics: measuring and testing – By light interference – Holography

Reexamination Certificate

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Reexamination Certificate

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07812959

ABSTRACT:
The present invention provides for a digital holographic microscope using a holographic interferometer and incorporating a TIR sample mount and microscopic imaging optics. The microscope uses phase shifting from frustrated internal reflection within a prism to measure nanometric distances. The invention also provides for a numerical reconstruction algorithm of an inclined surface of the object/prism.

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Marquet, P., Rappaz, B., Magistretti, P. J., Cuche, E., Emery, Y., Colomb, T., and Depeursinge, C.. 2005. “Digital Holographic Microscopy: A Noninvasive Contrast Imaging Technique Allowing Quantitative Visualization of Living Cells with Subwavelength Axial Accuracy.” Optic Letters. vol. 30, No. 5, pp. 468-470.

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