Device for electrofusion of cells

Details Device for electrofusion of cells Device for electrofusion of cells

1989-05-11

1991-04-16

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041831, 435173, 435273, 935 85, 935 89, 935 93, C12N 1300

Patent

active

050079956

ABSTRACT:
A device and a method for fusing biological cells. The device is formed by a chamber having a tube for containing a cell suspension and a cover for sealing the tube. The chamber is provided with a lower electrode having a smooth and flat surface for contacting the cells precipitating in the cell suspension, and an upper electrode opposite to the lower electrode. The lower electrode forms the inner bottom surface of the tube. The upper electrode is inserted in the cover to enter the tube. The chamber is centrifuged to form the layers of cells on the lower electrode at the bottom of the tube. Then, a predetermined ac voltage is applied across the upper and lower electrodes to fuse the cells efficiently. Such a device and method are useful for the production of monoclonal antibodies or giant cells.

REFERENCES:
patent: 4081340 (1978-03-01), Zimmermann et al.
patent: 4154668 (1979-05-01), Zimmermann et al.
patent: 4441972 (1984-04-01), Pohl
patent: 4476004 (1984-10-01), Pohl
patent: 4578168 (1986-03-01), Hofmann
patent: 4663292 (1987-05-01), Wong et al.
patent: 4822470 (1989-04-01), Chang

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