Process for the production of L-alanine dehydrogenase from 78-3

Details Process for the production of L-alanine dehydrogenase from 78-3 Process for the production of L-alanine dehydrogenase from 78-3

1989-09-26

1992-05-26

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

4352521, C12N 902, C12N 112

Patent

active

051167484

ABSTRACT:
A process for the production of L-alanine dehydrogenase which comprises culturing an L-alanine dehydrogenase producing microorganism belonging to the genus Sporolactobacillus in a nutrient medium and isolating thus-produced L-alanine dehydrogenase from the cultured mass. The microorganism is for example Sporolactobacillus sp. 78-3 FERM BP-2517 and mutants and variants thereof having the ability to produce L-alanine dehydrogenase in recoverable amounts. This strain is a thermophile which cannot grow at 40.degree. C. but can grow at 45.degree. C. and 52.degree. C. The strain can assimilate glucose and produce lactic acid and acetic acid.

REFERENCES:
patent: 3262862 (1966-07-01), Kitahara
"Thermostable Amino Acid Dehydrogenases: Applications and Gene Cloning", Trends in Biotechnology, vol. 7, No. 8, Aug. 1989, by T. Ohshima et al., pp. 210-214.
Nagata et al., Biochimie, 71(4), pp. 559-564, 1989.
Bergey's Manual of Systematic Bacteriology, vol. 2, p. 1127.

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