Method of assaying target substance

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 79, 435174, 435181, 435180, 435 6, 436501, 436524, C12Q 178, C12Q 168, C12Q 100, G01N 3353

Patent

active

051167336

ABSTRACT:
Disclosed is a method of assaying a target substance. In the method of the present invention, a complex of the target substance and a substance labelled with peroxidase is fixed to a hydrophobic membrane. Then hydrogen peroxide, an aromatic primary amine and a phenolic compound are contacted with the peroxidase on the membrane to generate and deposit color on the hydrophobic membrane. The deposited color is then measured so as to assay the target substance.

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patent: 4727024 (1988-02-01), Koocher et al.
patent: 4885250 (1989-12-01), Eveleigh et al.
patent: 4921791 (1990-05-01), Yamasaki et al.
White (1986) Medical Virology Academic Press, Orlando, p. 330.

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