Preparation of plasma or serum sample from whole blood

Details Preparation of plasma or serum sample from whole blood Preparation of plasma or serum sample from whole blood

1997-01-17

1999-03-02

Kim, John

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210645, 210651, 210653, 210654, 210723, 210767, 435 2, 436177, 436178, B01D 6100

Patent

active

058766057

ABSTRACT:
The method of the invention is for preparing a plasma or serum sample from whole blood and includes mixing an aqueous solution of an inorganic salt or an amino acid or salt thereof with the whole blood in an amount of 20% or less of the whole blood volume so that concentration of the inorganic salt or the amino acid or salt thereof becomes 10 to 200 .mu.mol/1 ml whole blood, and then, filtering the whole blood to remove blood cell components. According to the method of the invention, plasma separation from whole blood can be achieved efficiently, and recovered volume of plasma can be increased. By selecting reagent, good plasma which has no deviation in component viewpoint, and which is possible to measure in the same level accuracy as a plasma obtained by centurifuging, can be obtained through a simple filtration operation.

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Database WPI-Derwent Publications AN 90-295055 & JP 04 208 565 A-Fuji Photo Film Co. Ltd.
Infusionstherapie 19, 1992, pp. 187-189, Coden-1FTRE3, XP000671432-Taborshi, et al.-"Application of an Automated Plasma Filtration Device and a Blood Monitoring system for LDL Apheresis".
Vos Sang (1976), 31,4, pp. 289-295 Coden, Vosaad-XP000671437-Friedli, et al-"Studies on New Process Procedures in Plasma Fractionation on an Industrial Scale", III Removal of Precipitates by Filtration through a Horizontal Leaf Filter with Centrifugal Cleaning (Funda Filter) as an Alternative to Tubular-Bowl Centrifuges.

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