Multiple immunochemistry assays on an element

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007500, C435S007920, C435S007930, C435S007940, C435S007950, C435S960000, C435S969000, C435S971000, C435S973000

Reexamination Certificate

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07816090

ABSTRACT:
A method of analyzing a sample for one or more analytes of interest includes: providing an element having a base layer; a layer containing streptavidin; a spreading layer, wherein the streptavidin may or may not be in the spreading layer; providing immunocomponents and labeled immunoreactants which may be in the spreading layer or may be combined with the sample; dispensing the sample, and optionally the immunocomponents and a labeled immunoreactant onto the spreading layer at three or more areas, wherein each center of the three or more areas is substantially equidistance to the center formed by the three or more areas, and wherein each area contacts an adjacent area such that wash fluid flow in any direction will contact sample; washing the sample and label by directing the wash fluid at the center formed by the three or more areas, whereby the wash fluid equally flows over each of the three or more areas; and taking a measurement at each three or more areas to determine the presence or concentration of the one or more analyte. In a preferred embodiment, the element is a dry slide used on a diagnostic analyzer.

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Gosling, Immunoassays, Oxford University Press, 2000, pp. 7-15.
Randox Laboratories Ltd., Overview of Evidence®, Copyright Randox Laboratories, Aug. 2002.

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