Device and method for the detection and enumeration of...

Details Device and method for the detection and enumeration of... Device and method for the detection and enumeration of...

2007-06-21

2010-06-29

Beisner, William H (Department: 1797)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S034000, C435S261000, C435S288700

Reexamination Certificate

active

07745169

ABSTRACT:
A device and method simultaneously detects and enumerates two groups of microorganisms in a test sample, utilizing a single test container. In the container liquid growth media, a chromogenic substrate and a fluorogenic substrate are mixed with the test sample. The test container is incubated to allow bacterial growth and metabolism. Spectral changes of the substrates are dynamically detected using two external light sources aimed at a transparent section of the test container, and a single external photo detector. One light source operates in the visible band and the second in the long ultraviolet band. The two dynamic time patterns generated by the two substrates are analyzed in real time to determine the presence or absence of each microorganisms group and to enumerate their original concentrations in the test sample.

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Ruth Firstenberg-Eden, Debra L. Foti, Susan T. McDougal, Jackie Baker. “Optical instrument for the rapid detection of microorganisms in dairy porducts” Elsevier. International Dairy Journal vol. 12 2002 pp. 225-232.

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