Combinations of transfection lipids exhibiting increased...

Details Combinations of transfection lipids exhibiting increased... Combinations of transfection lipids exhibiting increased...

2004-10-04

2010-06-08

Kelly, Robert M (Department: 1633)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C435S325000, C436S071000

Reexamination Certificate

active

07732420

ABSTRACT:
The present invention provides optimized transfection reagents comprising mixtures of cationiclipoids. In particular, the present invention provides DNA delivery vehicles based on identifying the optimal hydrophobicity of novel cationic phospholipid derivatives that, alone or in combination, form complexes with DNA (lipoplexes) and exhibit enhanced transfection activity.

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Tarahovsky, Y., et al. “DNA Release from Lipoplexes by Anionic Lipids: Correlation with Lipid Mesomorphism, Interfacial Curvature, and Membrane Fusion”, Biophysical Journal, vol. 87, pp. 1054-1064 (Aug. 2004).
Pedroso de Lima, M., et al. “Cationic lipid-DNA complexes in gene delivery: from biophysics to biological applications”, Advanced Drug Delivery Reviews, vol. 47, pp. 277-294 (2001).
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MacDonald, R., et al. “Physical and Biological Properties of Cationic Triesters of Phosphatidylcholine”, Biophysical Journal, vol. 77, pp. 2612-2629 (Nov. 1999).
Faneca, H., et al. “Association of albumin or protamine to lipoplexes: enhancement of Transfection and resistance to serum”, The Journal of Gene Medicine, vol. 6, pp. 681-692 (2004).

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