Chemistry: analytical and immunological testing – Including sample preparation
Reexamination Certificate
2005-09-27
2010-02-16
Hill, Jr., Robert J (Department: 1797)
Chemistry: analytical and immunological testing
Including sample preparation
C436S008000, C436S080000, C436S086000, C436S164000, C436S166000, C436S169000, C436S905000, C435S006120, C204S456000, C204S462000
Reexamination Certificate
active
07662640
ABSTRACT:
A method for silver staining a gel on which biological substances such as nucleic acids and proteins are separated by electrophoresis, which comprises at least the following steps: (a) the step of immersing an untreated gel after the electrophoresis in an aqueous solution of sodium thiosulfate; (b) the step of immersing the gel obtained in the step (a) in a mixture of ethanol and an aqueous solution of sodium acetate; and (c) the step of immersing the gel obtained in the step (b) in a mixture of ethanol and an aqueous solution of silver nitrate.
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patent: 5064768 (1991-11-01), Ebata et al.
patent: 5492810 (1996-02-01), Caetano-Anolles et al.
patent: 02-080958 (1990-03-01), None
English language Abstract of JP 02-080958.
M. Swain et al., Electrophoresis, 1995, vol. 16, pp. 948-951.
F. Zalazar et al., Analytical Biochemistry, 2001, vol. 291, pp. 299-300.
A. Shevchenko et al., Anal. Chem., 1996, vol. 68, pp. 850-858.
J. X. Yan et al., Electrophoresis, 2000, vol. 21, pp. 3666-3672.
Gerido Dwan A
Greenblum & Bernstein P.L.C.
Hill, Jr. Robert J
Kyoto University
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