Method to treat collagenous connective tissue for implant...

Details Method to treat collagenous connective tissue for implant... Method to treat collagenous connective tissue for implant...

2006-01-19

2010-06-15

Tsang, Cecilia (Department: 1654)

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

C600S035000, C623S013170

Reexamination Certificate

active

07736845

ABSTRACT:
The invention relates to a method of treatment of collagenous connective tissue removed from a donor for implant into a recipient which is re-habited or re-colonized by host cells without an immune rejection and inflammatory reaction. After removal from the donor the tissue is trimmed and thereafter soaked in a cold stabilizing solution having a temperature range of 4 to 10 degrees centigrade. The tissue is then soaked at a predetermined temperature in a polyglycol, salt, hydrogen peroxide, and phosphate buffer first solution of predetermined quantities and concentrations and of sufficient ionic strength to permit ground substances to dissociate such that the collagen fibers remain stable. The tissue is then soaked in an alcohol and water solution at a predetermined temperature for a sufficient period of time to remove the residue of the first solution. Following the removal of the residue, the tissue is soaked at a predetermined temperature in a third solution of an anti-inflammatory agent, an anti-thrombic agent, alcohol, and water or sequentially in an anti-inflammatory agent, alcohol, and water solution, and then in an anti-thrombic agent, alcohol and water solution and thereafter stored.

REFERENCES:
patent: 6277555 (2001-08-01), Duran et al.
patent: 7008763 (2006-03-01), Cheung
patent: 2004/0057936 (2004-03-01), Cheung

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