Liquid purification or separation – Processes – Treatment by living organism
Reexamination Certificate
2008-08-20
2010-02-09
Barry, Chester T (Department: 1797)
Liquid purification or separation
Processes
Treatment by living organism
C210S903000, C435S262500
Reexamination Certificate
active
07658850
ABSTRACT:
The present invention provides a mixed bacterial culture, designated as the culture Atz Mix 1, which degrades atrazine at various temperatures (10° C. to 30° C.) in a wide range of atrazine concentrations (several ppb to ten thousand ppm), without formation of toxic metabolites. Atz Mix 1 is a stable mixed culture and includes catabolic genes trzN, atzB, atzC coding the enzymes for the degradation of atrazine to cyanuric acid, and the gene trzD coding the enzymes for subsequent opening of s-triazine ring. The invention further includes a microbiological method of degrading atrazine and other s-triazine compounds for remediation of atrazine-contaminated soils, even those rich in nitrogen, as well as for accelerating the process of atrazine mineralization in waste waters containing high concentrations of s-triazine compounds.
REFERENCES:
patent: 5429949 (1995-07-01), Radosevich et al.
patent: 5508193 (1996-04-01), Mandelbaum et al.
patent: 2003/0170879 (2003-09-01), McTavish
patent: 2004/0208706 (2004-10-01), Kukor et al.
patent: 2006/0257890 (2006-11-01), Minshull et al.
patent: 2007/0101461 (2007-05-01), Van Der Lelie et al.
Behki, Ram M, et al “Degradation of Atrazine, propazine, and Simazine by Rhodococcus Strain B-30” J. Agric Food Chem., pp. 1237-1241, 1994.
Martinez, Better, et al, “Complete Nucleotide Sequence and Organization of the Atrazine Catabolic Plasmid pADP-1 from Pseudomonas sp. Strain ADP” Journal of Bacteriology, pp. 5684-5697, Oct. 2001.
Strong, Lisa C., et al “Arthrobacter aurescens TC1, Metabolizes Diverse s-Trazine Ring Compounds”, Applied and Enviromental Microbiology, pp. 5973-5980, Dec. 2002.
Sparling, Graham, et al, “Atrazine miseralisation in New Zealand topsoils and subsoils: influence of edaphic factors and numbers of atrazine-degrading microbes”, Australian Journal of Soil Research, pp. 557-570, 1998.
de Souza, Mervyn L., “Atrazine Chlorohydrolase from Pseudomonas sp. Straid ADP: Gene Sequence, Enzyme Purification and Protein Characterization” Journal of Bacteriology, pp. 4894-4900, Aug. 1996.
Sadowsky, Michael J., et al, “AtzC is a New Member of the Amidohydrolase Protein Superfamily and Is Homologous to Other Atrazine-Metabolizing Enzymes” Journal of Bacteriology, pgs. 152-158, Jan. 1998.
Topp, Edward, et al, “Characterization of an Atrazine-Degrading Pseudaminobacter sp. Isolated from Canadian and French Agricultural Soils” Applied and Enviromental Microbiology, pp. 2773-2782, Jul. 2000.
Stipicevic, Sanja, et al, “Comparison of gas and high performace liquid chromatography with selective detection for determination of triazine herbicides and their degradation products extracted ultrasonically from soil” Journal of Separation Science, pp. 1237-1246, 2003.
Bouquard, Corinne, et al, “Dechlorination of Atrazine by Rhizobium sp. Isolate”, Applied and Environnental Microbiology, pp. 862-866, Mar. 1997.
Radosevich, Mark, et al, “Degradation and Mineralization of Atrazine by a Soil Bacterial Isolate” Applied and Enviromental Microbiology, pp. 297-302, Jan. 1995.
Behki, Ram M., et al “Degradation of Atrazine by Pseudomonas: N-Dealkylation and Dehalogenation of Atrazine and Its Metabolites” J. Agric. Food Chem, pp. 746-749, 1986.
Hrsak, D., et al, “Enrichment of linear alkylbenzenesuplhonate (LAS) degrading bacteria in continuous culture” Journal of Applied Bacteriology, pp. 413-422, 1982.
Gebendinger, N., et al, Inhibition of atrazne degradation by cyanazine and exogenous nitrogen in bacterial isolate M91-3, Appl Microbiol Biotechnol, pp. 375-381, 1999.
International Preliminary Report on Patentability, PCT/HR2007/000002, Feb. 29, 2008, 7 pages.
Rousseaux, Sandrine, et al, “Isolation and characterisation of new Gram-negative and Gram-positive atrazine degrading bacteria from different French soils” FEMS Microbiology, pp. 211-222, 2001.
Mandelbaum, Ralphi T., et al, “Isolation and Characterization of a Pseudomonas sp. That Mineralizes the s-Triazine Herbicide Atrazine” Applied and Enviromental Microbiology, pp. 1451-1457, Apr. 1995.
International Search Report, PCT/HR2007/000002, Jun. 22, 2007, 2 pages.
Kontchou, Clotaire Yanze, et al, “Mineralization of the Herbicide Atrazine in Soil Inoculated with a Pseudomonas Strain” J. Agric. Food Chem., pp. 2291-2294, 1995.
Ralebitso, T. Komang, et al “Microbial aspects of atrazine degradation in natural enviroments” Biodegeneration, pp. 11-19, 2002.
Garcia-Gonzales, Vicente, et al, “Regulation of the Pseudomonas sp. Strain Adp Cyanuric Acid Degradation Operon” Journal of Bacteriology, pgs. 155-167, Jan. 2005.
Smith D et al: “Cooperative catabolic pathways within an atrazine-degrading enrichment culture isolated from soil” Fems Microbiology Ecology, Elsevier, NL, vol. 53, No. 2, Jul. 1, 2005 (Jul. 1, 2005), pp. 265-273, XP004945377 ISSN: 0168-6496 abstract; figures 3,4 paragraph [02.2].
Stucki G et al: “Microbial atrazine mineralisation under carbon limited and denitrifying conditions” Water Research, Elsevier, Amsterdam, NL, vol. 29, No. 1, Jan. 1995 (Jan. 1995), pp. 291-296, XP004035522 ISSN: 0043-1354 abstract.
Boundy-Mills, Kyria L., et al, “The atzB Gene of Pseudomonas sp. Strain ADP Encodes the Second Enzyme of a Novel Atrazine Degradation Pathway”, Applied and Enviromental Microbiology, pp. 916-923, Mar. 1997.
Written Opinion of the International Searching Authority, PCT/HR2007/000002, Jun. 22, 2007, 6 pages.
Udikovic, et al., “Enrichment and Characterization of Atrazine Degrading Bacterial Communites”; Food Technol. Biotechnol. 41 (3) 211-217; 2003; 7 pages.
Havriluk Maja
Hrsak Dubravka
Barry Chester T
Rudjer Boskovic Institute
St. Onge Steward Johnston & Reens LLC
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