Method of DNA sequencing using cleavable tags

Details Method of DNA sequencing using cleavable tags Method of DNA sequencing using cleavable tags

2003-06-30

2010-10-19

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S077000, C435S078000, C536S024330, C536S025300, C536S027400

Reexamination Certificate

active

07816079

ABSTRACT:
The present invention provides novel systems for sequencing nucleic acid molecules using dNTPs that are 3′ end labeled with cleavable tags that block further extension and uniquely identify the bases to which they are attached. Removal of the tags liberates the 3′ ends of the extension products for further extension. In related embodiments, oligonucleotides containing sequence-related cleavable tags are employed in a ligation reaction to determine the sequence of a particular DNA sample.

REFERENCES:
patent: 4971903 (1990-11-01), Hyman
patent: 5302509 (1994-04-01), Cheeseman
patent: 5407779 (1995-04-01), Studier
patent: 5547839 (1996-08-01), Dower et al.
patent: 5552278 (1996-09-01), Brenner
patent: 6027890 (2000-02-01), Ness et al.
patent: 6087095 (2000-07-01), Rosenthal et al.
patent: 6268146 (2001-07-01), Shultz et al.
patent: 6514700 (2003-02-01), Singh
patent: 2002/0040873 (2002-04-01), Wahlberg et al.
patent: 2004/0033522 (2004-02-01), Fischer
patent: WO 96/27025 (1996-09-01), None
patent: WO 97/27331 (1997-07-01), None
patent: WO 98/13523 (1998-04-01), None
patent: WO 98/28440 (1998-07-01), None
patent: WO 99/02728 (1999-01-01), None
patent: WO 99/05315 (1999-02-01), None
patent: WO 99/66313 (1999-12-01), None
patent: WO 00/40750 (2000-07-01), None
patent: WO 00/43540 (2000-07-01), None
patent: WO 00/56455 (2000-09-01), None
European Search Report Dated: Nov. 4, 2004.
Prober, et al., “A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides” Science, 238: 336-341, 1987.
Sanger, et al., “DNA Sequencing with Chain-Terminating Inhibitors”, Proc. Natl. Acad. Sci. USA, 74(12): 5463-5467, 1977.

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