Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2003-12-03
2009-10-13
Landsman, Robert (Department: 1647)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S252300, C435S471000
Reexamination Certificate
active
07601513
ABSTRACT:
Methods for expressing a heteromeric taste receptor that responds to umami taste stimuli are provided. These methods comprise the co-expression of T1R1 and T1R3 nucleic acid sequences in a host cell that desirably also expresses a G protein that couples therewith, e.g., Gα15 and Gα16 or gustducin. In preferred embodiments, the host cells will be mammalian cells or Xenopus oocytes. These nucleic acid sequences are expressed constitutively or under inducible conditions. In preferred embodiments, the methods will yield mammalian cells that stably express a T1R1/T1R3 umami taste receptor under inducible conditions, e.g., HEK-293 cells that express Gα15.
REFERENCES:
patent: 5993778 (1999-11-01), Firestein et al.
patent: 6383778 (2002-05-01), Zuker et al.
patent: 6540978 (2003-04-01), Margolskee et al.
patent: 2002/0051997 (2002-05-01), Zuker et al.
patent: 2002/0094551 (2002-07-01), Adler
patent: 2002/0151052 (2002-10-01), Chaudhari et al.
patent: 2002/0160424 (2002-10-01), Adler et al.
patent: 2002/0168635 (2002-11-01), Zuker et al.
patent: 2003/0008344 (2003-01-01), Adler et al.
patent: 2003/0022278 (2003-01-01), Zuker et al.
patent: 2003/0022288 (2003-01-01), Zuker et al.
patent: 2003/0036089 (2003-02-01), Wei et al.
patent: 2003/0040045 (2003-02-01), Zuker et al.
patent: 2003/0054448 (2003-03-01), Adler et al.
patent: 2003/0166137 (2003-09-01), Zuker et al.
patent: 2004/0086877 (2004-05-01), Lal et al.
patent: 2005/0106571 (2005-05-01), Erlenbach et al.
patent: 2007/0105159 (2007-05-01), Erlenbach et al.
patent: WO 00/06592 (2000-02-01), None
patent: WO 00/06593 (2000-02-01), None
patent: WO 01/64882 (2001-09-01), None
patent: WO 01/66563 (2001-09-01), None
patent: WO 01/83749 (2001-11-01), None
patent: WO 03/025137 (2002-03-01), None
patent: WO 02/064631 (2002-08-01), None
patent: WO 03/001876 (2003-01-01), None
patent: WO 2005/015158 (2005-02-01), None
Extended European Search Report and Opinion for European Application No. 08075748.7, dated Nov. 21, 2008.
Damak, S., “Detection of Sweet and Umani Taste in the Absence of Taste Receptor Tlr3”, downloaded from the internet at: www.sciencemag.org, Jan. 9, 2009.
Wolfgang Bönigk, et al., “The Native Rat Olfactory Cyclic Nucleotide-Gated Channel is Composed of Three Distinct Subunits”, The Journal of Neuroscience, vol. 19, No. 136, p. 5332-5347, Jul. 1, 1999.
Jean-Pierre Montmayeur, et al., “A Candidate Taste Receptor Gene Near a Sweet Taste Locus”, Nature Neuroscience, vol. 4, No. 5, May 2001.
Michinori Kitagawa, et al., “Molecular Genetic Identification of a Candidate Receptor Gene for Sweet Taste”, Biochemical and Biophysical Research Communications, vol. 283, p. 236-242, 2001.
Marianna Max, et al., “Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac”, Nature Genetics, vol. 28, p. 58-63, May 2001.
Eduardo Sainz, et al., “Identification of a Novel Member of the T1R family of putative taste receptors”, Journal of Neurochemistry, vol. 77, p. 896-903, 2001.
Claire Johnson, et al., “The Effect of the Sweetness Inhibitor 2(-4-methoxyphenoxy) propanoic acid (sodium salt) (Na-PMP) on the taste of bitter-sweet stimuli”, Chemical Senses, vol. 19, No. 4, p. 349-358, 1994.
Sue C. Kinnamon and Thomas A. Cummings, “Chemosensory Transduction Mechanisms in Taste”, Annu. Rev. Physoil., vol. 54, p. 715-731, 1992.
Hoon, et al., “Putative Mammalian Taste Receptors: a Class of Taste-Specific GPCRs with Distinct Topographic Selectivity”, Cell, vol. 96, p. 541-551, Feb. 19, 1999.
Lindemann, “A Taste for Umami”, Nature Neuroscience, ol. 3, No. 2, p. 99-100, Feb. 2000.
Perruccio, et al., “Possible Role for gustducin in Taste Transduction in Hirudo Medicinalis”, Society for Neuroscience Abstracts, vol. 26, No. 1-2, Abstract No. 66.15, 2000.
Xiadong Li, et al., “Human Receptors for Sweet and umami taste”, Proceeding of the National Academy of Science, vol. 99, No. 7, p. 4692-4696, Apr. 2, 2002.
Database EMBL 'Online!, embl heidelberg; Acc#: Ac062024, Jun. 21, 2000.
E. Adler, et al., “A Novel Family of Mammalian Taste Receptors”, Cell, vol. 100, No. 6, p. 693-702, Mar. 17, 2000.
Guo, et al., “Protein Tolerance to Random Amino Acid change”, PNAS, vol. 101, No. 25, p. 9205-9210, Jun. 22, 2004.
Nelson, et al., “Mammalian Sweet Taste Receptors”, Cell, vol. 106, p. 381-390, Aug. 10, 2001.
Nelson,et al., “An Amino-acid Taste Receptor”, Nature, vol. 416, p. 199-202, Mar. 14, 2002.
Alexander, et al., “Altering the Antigenicity of proteins”, Proc. Natl. Acad. Sci. USA, vol. 89, p. 3352-3356, Apr. 1992.
Bowie, et al., “Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions”, Science, vol. 247, p. 13061310, Mar. 16, 1990.
Krautwurst, et al., “Identification of Ligands for Olfactory Receptors by Functional Expression of a Receptor Library”, Cell, vol. 95, p. 917-926, Dec. 23, 1998.
GenBank Accession No. AL139287, clone RP5-89003, Feb. 13, 2000.
GenBank Accession No. AA907022, May 19, 1998.
Adler Jon Elliot
Echeverri Femando
Li Xiaodong
O'Connell Shawn
Staszewski Lena
Hunton & Willims LLP
Landsman Robert
Senomyx Inc.
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