Transformed mammalian cells capable of expressing cecropin b

Details Transformed mammalian cells capable of expressing cecropin b Transformed mammalian cells capable of expressing cecropin b

1995-05-25

1996-09-17

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524021, 4351723, 4353201, 4352523, 536 231, 536 235, 935 9, 935 10, 935 11, 935 22, 935 29, 935 24, 935 34, 935 70, 935 71, 935 72, 935 73, C12N 510, C12N 1585

Patent

active

055567827

ABSTRACT:
Novel means have been discovered for increasing the resistance of a mammalian host (including humans) to diseases caused by intracellular bacteria, protozoa, and viruses. Novel means have also been found for treating tumors. Augmentation of the host's defenses against infectious diseases or tumors is achieved by "arming" the host's cells with a gene encoding the lytic peptide cecropin B. The host's own leukocytes, other cells involved in resistance to infection, or other cells are transformed, and expression of the gene is induced when needed to combat pathogens. Transfection of hematopoietic stem cells with the cecropin B gene will enhance disease resistance in mammals; and transfection of TIL (tumor infiltrating lymphocytes) cells or other cells can be used in the treatment of tumors. The transformed cells have the ability to produce and secrete a broad spectrum chemotherapeutic agent which has a systemic effect on certain pathogens, particularly pathogens that might otherwise evade or overcome host defenses. The peptide's expression is preferably induced only in areas of infection, where it will most effectively augment the host's defense systems. Expression of the exogenous gene does not damage a healthy recipient cell.

REFERENCES:
patent: 5028530 (1991-07-01), Lai et al.
patent: 5206154 (1993-04-01), Lai et al.
Klysten etal. 1990 EmBO J. 9, 217-224.
Pelham etal. 1982. "DNA Sequences Required for Transcriptional regulation of the Drosophila hsp 70 Heat Shock 9 cm in Monkey Cells and Xenopus Oocytes", in: (Schlessinger etal. eds.). Heat Shock. From Bacteria to Man, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. pp. 43-48.
Asubel etal. 1988. in: Cumont Protocols in Molecular Biology., Greene Publ. Assoc. & Wiley Inter Sci. (John Wiley & Sons). New York, pp. 9.0.3-9.0.4.
Xanthopoulas et al. , "The structure of the gene for cecropin B, an antibacterial immune protein from Hyalophora cecropia, " Eur. J. Biochem., vol. 172, pp. 371-376 (1988).
Kaling et al. , "Liver-Specific Gene Expression: A-Activator-Binding Site, a Promoter Module Present in Vitellogen and Acute-Phase Genes," Mol. Cell. Biol., vol. 11, pp. 93-101 (1991).
Boman et al., "Cell-free immunity in Cecropia," Eur. J. Biochem., vol. 201, pp. 23-31 (1991).
Beck et al., "Invertebrate Cytokines III: Invertebrate Interleukin-1-like Molecules Stimulate Phagocytosis by Tunicate and Echinoderm Cells," Cellular Immunology, vol. 146, pp. 284-299 (1993).
Caput et al., "Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators," Proc. Natl. Acad. Sci. USA, vol. 83, pp. 1670-1674 (1986).

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