Method and apparatus for nested polymerase chain reaction (PCR)

Details Method and apparatus for nested polymerase chain reaction (PCR) Method and apparatus for nested polymerase chain reaction (PCR)

1994-08-18

1996-09-17

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 5, 435177, 536 243, 536 031, 536 032, 536 033, C12P 1934, C12Q 168, C12Q 170, C12N 1102

Patent

active

055567738

ABSTRACT:
A nested polymerase chain reaction (PCR) performed in a single reaction tube that remains closed after the reaction mixtures for each amplification have been introduced therein. The reaction mixture for the second PCR amplification is sequestered and preserved in an upper portion of the single, closed reaction tube during the first amplification, and subsequently introduced into the reaction space containing the end product of the first PCR amplification, without opening the reaction tube.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5304487 (1994-04-01), Wilding et al.
patent: 5314809 (1994-05-01), Erlich et al.
patent: 5340728 (1994-08-01), Grosz et al.
patent: 5364591 (1994-11-01), Green et al.
patent: 5382511 (1995-01-01), Stapleton et al.
patent: 5413924 (1995-05-01), Kosak et al.
Yourno, "A Method for Nested PCR with Single Closed Reaction Tubes", PCR Methods and Applications, Aug. 15, 1992, pp. 61-65.
Mullis et al., "Specific Synthesis of DNA in Vitro Via a Polymerase-Catalyzed Chain Reaction", Methods in Enzymology, Academic Press, vol. 155, 1987, pp. 335-350.

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