Brachyspira hyodysenteriae vaccine

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Reexamination Certificate

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07569682

ABSTRACT:
The present invention relates to nucleic acid sequences encoding a 30 kDBrachyspira hyodysenteriaelipoprotein and to parts of such nucleic acid sequences that encode an immunogenic fragment of such lipoproteins, and to DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof. The invention also relates to a 30 kDBrachyspira hyodysenteriaelipoprotein and immunogenic parts thereof encoded by such sequences. Furthermore, the present invention relates to vaccines comprising such nucleic acid sequences and parts thereof, DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof, lipoproteins or immunogenic parts thereof and antibodies against such lipoproteins or immunogenic parts thereof. Also, the invention relates to the use of said lipoproteins in vaccines and for the manufacture of vaccines. Moreover, the invention relates to the use of said nucleic acid sequences, lipoproteins or antibodies for diagnostic or vaccination purposes. Finally the invention relates to diagnostic kits comprising such a nucleic acid, lipoprotein or antibodies against such lipoprotein.

REFERENCES:
patent: 2004/0186280 (2004-09-01), Hampson et al.
patent: 2005/0163791 (2005-07-01), Adler et al.
patent: 2006/0057151 (2006-03-01), Hampson et al.
National Center for Biotechnology Information (NCBI;www.ncbi.nlm.nih.gov) Accession No. AAR98524, May 9, 2005.
European Molecular Biology Laboratory—European Bioinformatics Institute (EMBL-EBI;www.ebi.ac.uk/embl) Accession No. AY512344, Jan. 25, 2004.
Cullen, P.A. et al, “Characterization of a locus encoding four paralogous outer membrane lipoproteins ofBrachyspira hyodysenteriae,” Microbes and Infection, vol. 5, p. 275-283 (2003).
Lee, B.J. et al., “Identification of the gene encoding BmpB, a 30 kDa outer envelope lipoprotein . . . ,” Veterinary Microbiology, vol. 76, p. 245-257 (2000).
Haake, D.A., “Spirochaetal lipoproteins and pathogenesis,” Microbiology, vol. 146, p. 1491-1504 (2000).
Chatfield, S.N. et al., “Identification of the Major Antigens ofTreponema hyodysenteriaeand Comparison with Those of Treponema innocens,” Infection and Immunity, vol. 56, No. 5, p. 1070-1075 (May 1998).
Lee, B.J. et al., “Production and characterization of a monoclonal antibody toSerpulina hyodysenteriae,” FEMS Microbiology Letters, vol. 136, p. 193-197 (1996).
Thomas, W. et al., “A 16-Kilodalton Lipoprotein of the Outer Membrane ofSerpulina(Treponema)hyodysenteriae,” Infection and Immunity, vol. 60, No. 8, p. 3111-3116, (Aug. 1992).

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