Process for producing N-acetylneuraminic acid

Details Process for producing N-acetylneuraminic acid Process for producing N-acetylneuraminic acid

2003-01-09

2009-08-25

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S004000, C435S006120, C435S041000, C435S069100, C435S183000, C435S193000, C435S200000, C435S252300, C435S262000, C435S320100, C530S350000, C536S023200, C536S023400, C536S023500, C536S023700

Reexamination Certificate

active

07579175

ABSTRACT:
The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.

REFERENCES:
patent: 5071750 (1991-12-01), Kragl et al.
patent: 5665574 (1997-09-01), Tsukada et al.
patent: 6-197778 (1994-07-01), None
patent: 10-4961 (1998-01-01), None
patent: 94/29476 (1994-12-01), None
Vann(a) et al. (Glycobiology , 1997, vol. 7(5):697-701).
Vann(b) et al. (J. Biol. Chem., 1987, vol. 262(36):17556-62).
Maru et al. (Carbohydrate Research, 1998, vol. 306:575-578).
Zhu, et al., “The effect ofpflgene knockout on the metabolism for optically pure . . . ”,App. Microbiol. Biotech., vol. 64 (2004), pp. 367-375.
Martin, et al., “A facile procedure for the isolation of . . . ”, Carbohydrate Research, vol. 56 (1977), pp. 423-425.
Uchida, et al., “Distribution of Neuraminidase inArthrobacter. . . ”, J. Biochem., vol. 82 (1977), pp. 1425-1433.
Kim, et al., “Enzymes in Carbohydrates Synthesis:N-Acetylneuraminic. . . ”, J. Am. Chem. Soc., vol. 110 (1988), pp. 6481-6486.
Simon, et al., “Synthesis of CMP-NeuAc fromN-Acetylglucosamine. . . ”, J. Am. Chem. Soc., vol. 110 (1988), pp. 7159-7163.
Kragl, et al., “Enzymatic Two-step Synthesis of N-Acetylneuraminic”, Agnew. Chem. Int. Ed. Engl., vol. 30, No. 7 (1991), pp. 827-828.
Maru, et al., “Simple and large-scale production of N-acetylneuraminic acid . . . ”, Carbohydrate Research, vol. 306 (1998), pp. 575-578.
Vann, et al., “Purification and characterization of the . . . ”, Glycobiology, vol. 7, No. 5 (1997), pp. 697-701.
Ohta, et al., “Complete nucleotide sequence of theE. coli-acetylneuraminate lyase”, Nucleic Acids Research, vol. 13, No. 24 (1985), pp. 8843-8852.
Annunziato, et al., “Nucleotide Sequence and Genetic Analysis . . . ” Journal of Bacteriology, vol. 177, No. 2 (1995), pp. 312-319.
Littlechild, et al., “A New Method for the Purification of 30S . . . ”, Biochemistry, vol. 17, No. 16 (1978), pp. 3363-3369.
Maru, et al., “Molecular Cloning and Identification of . . . ”, The Journal of Biological Chemistry, vol. 271, No. 27 (1996), pp. 16294-16299.
Yokoto, et al., “Pyruvic Acid Production by an F1—ATPase-defective Mutant . . . ”, Biosci. Biotech. Biochem., vol. 58, No. 12 (1994), pp. 2164-2167.
Mahmoudian, et al., An efficient process for production of . . . , Enzyme and Microbial Technology, vol. 20 (1997), pp. 393-400.
Stäsche, et al., “A Bifunctional Enzym Catalyzes . . . ”, J. Biol. Chem., vol. 272, No. 39 (1997), pp. 24313-24318.
Stäsche, et al., J. Biol. Chem., vol. 272, No. 39 (1997), pp. 24319-24324.
Vann, et al., “Purification Properties, and Genetic Location of . . . ”, J. Biol. Chem., vol. 262, No. 36 (1987), pp. 17556-17562.
Vann, et al., “Purification and Characterization of the . . . ” Glycobiology, vol. 7, No. 5 (1997), pp. 697-701.
Warren, et al., “The Biosynthesis of N-Acetylneuraminic Acid”, Biochem. Biophys. Research Communs, vol. 4, No. 3 (1961), pp. 232-235.
Kragl, et al., “N-Acetylneuraminic Acid From a Rare Chemical from Natural Sources . . . ”, Ann. N.Y. Acad. Sci (1995), pp. 300-305.
Vann, et al., “Purification and Characterization of theEscherichia coliK1neuBgene product . . . ”, Glycobiology (1997), vol. 7, No. 5, pp. 697-701.
Chemical Abstract, Database Accession No. XP-00218097, Kragl, “Reaction technology for Continuous enzymic . . . ”, (1992).

Affiliated with

Also associated with

Rating

Process for producing N-acetylneuraminic acid does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for producing N-acetylneuraminic acid, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for producing N-acetylneuraminic acid will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4120733