Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule
Reexamination Certificate
2002-10-30
2009-11-24
Mehta, Ashwin (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide encodes an inhibitory rna molecule
C435S468000
Reexamination Certificate
active
07622633
ABSTRACT:
A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods.
REFERENCES:
patent: 3931397 (1976-01-01), Harnden
patent: 4130641 (1978-12-01), Ts'o et al.
patent: 4283393 (1981-08-01), Field
patent: 4469863 (1984-09-01), Ts'o et al.
patent: 4511713 (1985-04-01), Miller et al.
patent: 4605394 (1986-08-01), Skurkovich
patent: 4766072 (1988-08-01), Jendrisak
patent: 4795744 (1989-01-01), Carter
patent: 4820696 (1989-04-01), Carter
patent: 4945082 (1990-07-01), Carter
patent: 4950652 (1990-08-01), Carter
patent: 4963532 (1990-10-01), Carter
patent: 5024938 (1991-06-01), Nozaki
patent: 5034323 (1991-07-01), Jorgensen et al.
patent: 5063209 (1991-11-01), Carter
patent: 5091374 (1992-02-01), Carter
patent: 5107065 (1992-04-01), Shewmaker et al.
patent: 5132292 (1992-07-01), Carter
patent: 5173410 (1992-12-01), Ahlquist
patent: 5190931 (1993-03-01), Inouye
patent: 5194245 (1993-03-01), Carter
patent: 5208149 (1993-05-01), Inouye
patent: 5258369 (1993-11-01), Carter
patent: 5272065 (1993-12-01), Inouye
patent: 5365015 (1994-11-01), Grierson et al.
patent: 5453566 (1995-09-01), Shewmaker et al.
patent: 5514546 (1996-05-01), Kool
patent: 5530190 (1996-06-01), Grierson et al.
patent: 5578716 (1996-11-01), Szyf et al.
patent: 5593973 (1997-01-01), Carter
patent: 5624803 (1997-04-01), Noonberg
patent: 5631148 (1997-05-01), Urdea
patent: 5643762 (1997-07-01), Ohshima et al.
patent: 5674683 (1997-10-01), Kool
patent: 5683985 (1997-11-01), Chu
patent: 5683986 (1997-11-01), Carter
patent: 5691140 (1997-11-01), Noren et al.
patent: 5693773 (1997-12-01), Kandimalla
patent: 5712257 (1998-01-01), Carter
patent: 5738985 (1998-04-01), Miles
patent: 5739309 (1998-04-01), Dattagupta
patent: 5747338 (1998-05-01), Giese
patent: 5795715 (1998-08-01), Livache
patent: 5808036 (1998-09-01), Kool
patent: 5850026 (1998-12-01), DeBonte
patent: 5874555 (1999-02-01), Dervan
patent: 5906980 (1999-05-01), Carter
patent: 5908779 (1999-06-01), Carmichael
patent: 5958718 (1999-09-01), Carter
patent: 5972704 (1999-10-01), Draper et al.
patent: 6010908 (2000-01-01), Gruenert et al.
patent: 6022863 (2000-02-01), Peyman
patent: 6130206 (2000-10-01), Carter
patent: 6133024 (2000-10-01), Helene
patent: 6136601 (2000-10-01), Meyer, Jr. et al.
patent: 6291504 (2001-09-01), Nugiel
patent: 6369038 (2002-04-01), Blumenfeld
patent: 6372965 (2002-04-01), Lightner
patent: 6506559 (2003-01-01), Fire
patent: 6531647 (2003-03-01), Baulcombe
patent: 6573099 (2003-06-01), Graham
patent: 6635805 (2003-10-01), Baulcombe
patent: 2003/0061626 (2003-03-01), Plaetinck et al.
patent: 2004/0138168 (2004-07-01), Satishchandran
patent: 199536778 (1995-09-01), None
patent: 199929163 (1999-03-01), None
patent: 200195225 (2001-11-01), None
patent: 2012312 (1990-03-01), None
patent: 2370628 (2000-04-01), None
patent: 00806369 (2000-04-01), None
patent: 0318281 (1989-05-01), None
patent: 0325018 (1989-07-01), None
patent: 0347501 (1989-12-01), None
patent: 0213921 (1990-08-01), None
patent: 0495831 (1990-09-01), None
patent: 0286224 (1992-11-01), None
patent: 0350151 (1994-03-01), None
patent: 0303516 (1994-07-01), None
patent: 0306347 (1995-05-01), None
patent: 0465572 (1995-06-01), None
patent: 0281380 (1995-11-01), None
patent: 0308066 (1995-12-01), None
patent: 0300680 (1996-09-01), None
patent: 983370 (2003-09-01), None
patent: 9710475.6 (1997-07-01), None
patent: 2353282 (2001-02-01), None
patent: 2362885 (2001-12-01), None
patent: 2377221 (2004-08-01), None
patent: 09-227413 (1997-09-01), None
patent: WO 90/14090 (1989-05-01), None
patent: WO 90/12094 (1990-10-01), None
patent: WO 90/12488 (1990-11-01), None
patent: WO 91/05559 (1991-05-01), None
patent: WO 91/18611 (1991-12-01), None
patent: WO 92/18522 (1992-10-01), None
patent: WO 93/23569 (1993-11-01), None
patent: WO 94/01550 (1994-01-01), None
patent: WO 95/03406 (1995-02-01), None
patent: WO 95/15378 (1995-06-01), None
patent: WO 95/18223 (1995-07-01), None
patent: WO 95/18854 (1995-07-01), None
patent: WO 95/34668 (1995-12-01), None
patent: WO 96/35706 (1996-11-01), None
patent: WO 97/10360 (1997-03-01), None
patent: WO 97/11170 (1997-03-01), None
patent: WO 97/44450 (1997-11-01), None
patent: WO 98/05770 (1998-02-01), None
patent: WO 98/53083 (1998-05-01), None
patent: WO 98/53083 (1998-11-01), None
patent: WO 99/32619 (1999-07-01), None
patent: WO 99/49029 (1999-09-01), None
patent: WO 99/53050 (1999-10-01), None
patent: WO 99/61631 (1999-12-01), None
patent: WO 00/01846 (2000-01-01), None
patent: WO 00/63364 (2000-10-01), None
patent: WO 01/070949 (2001-09-01), None
patent: WO 04/022748 (2004-03-01), None
Yu et al., Plant Cell Rep., 2003, vol. 22, pp. 167-174.
Horiguchi, G., Different., 2004, vol. 72, pp. 65-73.
Kumagai et al., Proc. Natl. Acad. Sci., USA, 1995, vol. 92, pp. 1679-1683.
Holtorf et al., Plant Mol. Biol., 1995, vol. 29, pp. 637-646.
Klein et al. (Nature, 1987, vol. 327, pp. 70-73.
Brisson et al., Nature, 1984, vol. 310, pp. 511-514.
McCabe et al., Biotech., 1988, vol. 6, pp. 923-926.
Metzlaff et al., Cell, Mar. 21, 1997, vol. 88, pp. 845-854.
van der Meer et al., Plant Mol. Biol., 1990, vol. 15, pp. 95-109.
Grayburn et al., Plant Cell Rep., 1995, vol. 14, pp. 285-289.
Cameron et al. (1991), “Inhibition of gene expression by a short sense fragment,” Nucl Acids Res 19(3):469-475.
Agrawal, “Antisense Oligonucleotides: Towards Clinical Trials” (1996),Tibtech14: 376-387.
Anderson, “Human Gene Therapy” (1998),Nature, 392: 25-30.
Branch, “A Good Antisense Molecule Is Hard to Find” (1998),TIBS23: 45-50.
Clemens et al., Use of Double-Stranded RNA Interference inDrosophilaCell Lines to Dissect Signal Transduction Pathways (1999),PNAS97: 6499-6503.
Mercola and Cohen, “Antisense Approaches to Cancer Gene Therapy” (1995),Cancer Gene Therapy2: 47-59.
Sharp, “RNAi and Double-Strand Rna” (1999),Genes&Development13: 139-141.
Verna and Somia, “Gene Therapy—Promises, Problems and Prospects” (1997),Nature389: 239-242.
Bevec et al., “Constitutive expression of chimeric neo-Rev response element transcripts suppresses HIV-1 replication in human CD4+ T lymphocytes” (1994)Hum Gene Ther5:193-201.
Cohli et al., “Inhibition of HIV-1 multiplication in a human CD4+ lymphocytic cell line expressing antisense and sense RNA molecules containing HIV-1 packaging signal and Rev response element(s)” (1994)Antisense Res Dev. 4:19-26.
Dorer et al., “Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila” (1994)Cell77:993-1002.
Dorer et al., “Transgene repeat arrays interact with distant heterochromatin and cause silencing in cis and trans” (1997)Genetics147:1181-1190.
Fraser et al., “Effects of c-myc first exons and 5′ synthetic hairpins on RNA translation in oocytes and early embryos of Xenopus laevis”(1996)Oncogene12:1223-1230.
Good et al., “Expression of small, therapeutic RNAs in human cell nuclei” (1997)Gene Therapy4:45-54.
Kozak “Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs” (1989)Mol Cell Biol9:5134-5142.
Liebhaber et al., “Translation inhibition by an mRNA coding region secondary structure is determined by its proximity to the AUG initiation codon” (1992)J Mol Biol226:609-621.
Lingelbach & Dobberstein “An extended RNA/RNA duplex structure within the coding region of mRNA does not block translational elongation” (1998)Nucleic Acids Res16:3405-3414.
Lisziewicz et al., “Inhibition of human immunodeficiency virus type 1 replication
Driver Samuel E.
Fire Andrew
Kostas Stephen
Mello Craig C.
Montgomery Mary
Carnegie Institution of Washington
Mehta Ashwin
Morgan & Lewis & Bockius, LLP
LandOfFree
Genetic inhibition by double-stranded RNA does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Genetic inhibition by double-stranded RNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genetic inhibition by double-stranded RNA will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4120292