Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2003-07-24
2009-02-03
Kubelik, Anne R (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C800S287000, C800S298000
Reexamination Certificate
active
07485771
ABSTRACT:
Amino acid and nucleotide sequences relating to the glutamate dehydrogenase (GDH) enzyme are described. The GDH enzymes described herein were discovered in the algaChlorella sorokinianain the form of seven different inducible isoenzymes. These isoenzymes are found in the algae as chloroplast-localized hexamers composed of alpha- and beta-subunits. Plants transformed with nucleotide sequences encoding the alpha- or beta-subunits of the enzyme show improved properties, for example, increased growth and improved stress tolerance. A heterohexamer having both α- and β-subunits can have higher aminating:deaminating activity ratio than α-homohexamers or β-homohexamers.
REFERENCES:
patent: 5879941 (1999-03-01), Schmidt et al.
patent: 5998700 (1999-12-01), Lightfoot et al.
patent: 6084153 (2000-07-01), Good et al.
patent: 6329573 (2001-12-01), Lightfoot et al.
patent: WO 9509911 (1995-04-01), None
Oliveira et al, 1997, Plant Physiol. Biochem. 35:185-198.
Chavez et al, 1995, Plant Mol. Biol. 28:173-188.
Long et al, 1994, Plant Physiol. 105:115.
Gupta et al, 1982, Mol. Gen. Genet. 188:378-383.
Cock, J.M. et al. (1991) “A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) inChlorella sorokiniana” Plant Moleclular Biology 17:1023-1044.
Bascomb, N.F. et al. (1987) “Different Rates of Synthesis and Degradation of Two Chloroplastic Ammonium-inducible NADP-Specific Glutamate Dehydrogenase Isoenzymes during Induction and Deinduction ofChlorella sorokinianaCells” Plant Physiol. 83:85-91.
Bascomb, N.F., R. R. Schmidt (1987) “Purification and Partial Kinetic and Physical Characterization of Two Chloroplast-Localized NADP-Specific Glutamate Dehydrogenase Isoenzymes and Their Preferential Accumulation inChlorella sorokinianaCells Cultured at Low or High Ammonium Levels” Plant Physiol. 83:75-84.
Prunkard, D.E. et al. (1986) “Effect of Different Carbon Sources on the Ammonium Induction of Different Forms of NADP-Specific Glutamate Dehydrogenase inChlorella sorokinianaCells Cultured in the Light and Dark” Plant Physio. 81:413-422.
Yeung, A.T. et al. (1981) “Purification of an Ammonium-Inducible Glutamate Dehydrogenase and the Use of its Antigen Affinity Column-Purified Antibody in Specific Immunoprecipitation and Immunoadsorption Procedures” Analytical Biochemistry 110:216-228.
Meredith, M.J. et al. (1978) “Physical and Kinetic Properties of the Nicontinamide Adenine Dinucleotide-specific Glutamate Dehydrogenase Purified fromChlorella sorokiniana” Plant Physio. 61:967-974.
Srivastava, H.S., R. P. Singh (1987) “Role and Regulation of L-Glutamate Dehydrogenase Activity in Higher Plants” Phytochemistry 26(3):597-610.
Prunkard, D. E. et al. (1986) “Evidence for Chloroplastic Localization of an Ammonium-Inducible Glutamate Dehydrogenase and Synthesis of its Subunit from a Cytosolic Precursor-Protein inChlorella sorokiniana” Plant Physio. 81:349-355.
Wallsgrove, R.M. et al. (1987) “Barley Mutants Lacking Chloroplast Glutamine Synthetase-Biochemical and Genetic Analysis” Plant Physio. 83:155-158.
Miflin, B.J. P. J. Lea (1976) “The Pathway of Nitrogen Assimilation in Plants” Phytochemistry 15.873-885.
“Niotimasmide Adenine Di Nucleotide Glutamate Dehydrogenase ObtainCholerellaCell Buffer Extract Two Stage Chromatography Phosphate Buffer Elution” (1982) Biochem. Inst., abstract only.
Napoli et al. Introduction of a chimeric chalcone synthase gene into petunia results lin reversible co-suppression of homologous genes in trans. The Plant Cell. 2:279-289 (1990).
Bascomb, N.F. et al. (1985) “Specific Polysome Immunoadsorption to Purify an Ammonium-Inducible Glutamate Dehydrogenase MRNA fromChlorella sorokinianaand synthesis of Full Length Double-Stranded cDNA from the Purified MRNA” Plant Physio. 81:527-532.
Miller, P. W. et al. (1994) “Transcription initiation site of a NADP-specific glutamate dehydrogenase gene and potential use of is promoter region to express foreign genes in ammonium-culturedChlorella sorokinianacells” Journal of Applied Phycology 6:211-223.
Meredith, M. J., R.R. Schmidt (1991) “NAO-Specific glutamate dehydrogenase isoenzyme localized in mitochondria of nitrate-culturedChlorella sorokiniancells” Plant Physio. 10:67-71.
Mehta, R.A. et al., Engineered polyamine accumulation in tomato enhances phytonutrient content, juice quality, and vine life Nature Biotechnology, Jun. 2002, vol. 20, 613-618.
Park, S.M. et al., Elucidation of anaplerotic pathways inCorynebacterium glutamicum, Appl. Microbiol Biotechnol, 1997, vol. 47, 430-440.
Miller Philip
Schmidt Robert R.
Kubelik Anne R
Saliwanchik Lloyd & Saliwanchik
University of Florida Research Foundation Inc.
LandOfFree
Polypeptides and polynucleotides relating to the α-... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Polypeptides and polynucleotides relating to the α-..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polypeptides and polynucleotides relating to the α-... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4112320