Methods of generating chimeric adenoviruses and uses for...

Details Methods of generating chimeric adenoviruses and uses for... Methods of generating chimeric adenoviruses and uses for...

2004-06-15

2009-02-17

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S455000, C435S456000, C435S325000, C435S366000, C424S093100, C424S093200, C424S093600

Reexamination Certificate

active

07491508

ABSTRACT:
A method for providing an adenovirus from a serotype which does not grow efficiently in a desired cell line with the ability to grow in that cell line is described. The method involves replacing the left and right termini of the adenovirus with the corresponding termini from an adenovirus which grow efficiently in the desired cell line. At a minimum, the left terminus spans the (5′) inverted terminal repeat, the left terminus spans the E4 region and the (3′) inverted terminal repeat. The resulting chimeric adenovirus contains the internal regions spanning the genes encoding the penton, hexon and fiber from the serotype which does not grow efficiently in the desired cell. Also provided are vectors constructed from novel simian adenovirus sequences and proteins, host cells containing same, and uses thereof.

REFERENCES:
patent: 5712136 (1998-01-01), Wickham et al.
patent: 5846782 (1998-12-01), Wickham et al.
patent: 5856152 (1999-01-01), Wilson et al.
patent: 5871982 (1999-02-01), Wilson et al.
patent: 5922315 (1999-07-01), Roy
patent: 5965541 (1999-10-01), Wickman et al.
patent: 6001557 (1999-12-01), Wilson et al.
patent: 6057155 (2000-05-01), Wickham et al.
patent: 6083716 (2000-07-01), Wilson et al.
patent: 6127525 (2000-10-01), Crystal et al.
patent: 6153435 (2000-11-01), Crystal et al.
patent: 6203975 (2001-03-01), Wilson et al.
patent: 6251677 (2001-06-01), Wilson et al.
patent: 6329190 (2001-12-01), Wickham et al.
patent: 6387368 (2002-05-01), Wilson et al.
patent: 6465253 (2002-10-01), Wickham et al.
patent: 6576456 (2003-06-01), Wickham et al.
patent: 6649407 (2003-11-01), Wickham et al.
patent: 7247472 (2007-07-01), Wilson
patent: 7291498 (2007-11-01), Roy
patent: 2004/0171807 (2004-09-01), Gao et al.
patent: 2004/0241181 (2004-12-01), Ertl et al.
patent: 2005/0069866 (2005-03-01), Wilson et al.
patent: WO 96/13598 (1996-05-01), None
patent: WO 00/03029 (2000-01-01), None
patent: WO 03/000283 (2003-01-01), None
patent: WO 03/000851 (2003-01-01), None
patent: WO 03/046124 (2003-06-01), None
Wu et al., “Construction and characterization of adenovirus serotype 5 packaged by serotype 3 hexon”, J Virol., Dec. 2002;76(24):12775-82.
Roy et al., “Circumvention of immunity to the adenovirus major coat protein hexon”, J Virol., Aug. 1998;72(8):6875-9.
Gall et al., “Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype”, J Virol., Dec. 1998;72(12):10260-4.
Gall et al., “Adenovirus type 5 and 7 capsid chimera: fiber replacement alters receptor tropism without affecting primary immune neutralization epitopes”, J Virol., Apr. 1996;70(4):2116-23.
Wohlfart, C., “Neutralization of adenoviruses: kinetics, stoichiometry, and mechanisms”, J Virol., Jul. 1988;62(7):2321-8.
Youil et al., “Hexon gene switch strategy for the generation of chimeric recombinant adenovirus”, Hum Gene Ther., Jan. 20, 2002;13(2):311-20.
Farina et al., “Replication-defective vector based on a chimpanzee adenovirus”, J Virol., Dec. 2001;75(23):11603-13.
Stevens, D., “SA18 (Simian adenovirus 18) ATCC VR-943”, American Type Culture Collection Catalogue of Strains II: Viruses and Antisera, 1983, p. 227: paragraph 1.
Roy et al., “Characterization of a family chimpanzee adenoviruses and development of molecular clones for gene transfer vectors”, Hum Gene Ther., May 2004;15(5):519-30.
International Search Report for PCT/US2004/016614 (WO 2005/001103 A2).
Written Opinion for PCT/US2004/016614 (WO 2005/001103 A2).
Roy et al., “Novel Chimeric Adenovirus Vaccine Vectors”, 7thAnnual Meeting of the American Society of Gene Therapy [Jun. 2-6, 2004], Abstract 1016.
Roy et al., “Use of Chimeric Adenoviral Vectors to Assess Capsid Neutralization Determinants”, 7thAnnual Meeting of the American Society of Gene Therapy [Jun. 2-6, 2004], Abstract 128.
Horwitz, “Adenoviridae and Their Replication”, Virology, 2d ed., 1990, pp. 1679-1721.
Roy et al., “Use of chimeric adenoviral vectors to assess capsid neutralization determinants”, Virology, Mar. 15, 2005;333(2):207-14.
Freimuth et al, Codon Insertion Mutants of the Adenovirus Terminal Protein, Proc. Nat'l Acad. Sci. USA, vol. 83, pp. 7816-7820, (Oct. 1986).

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