Ligand/binding partner bio-labeling systems

Details Ligand/binding partner bio-labeling systems Ligand/binding partner bio-labeling systems

2005-09-01

2009-08-18

Burkhart, Michael (Department: 1633)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S007600, C435S007710, C435S025000

Reexamination Certificate

active

07575866

ABSTRACT:
The present invention relates to methods and compositions which utilize binding relationships between detector molecules and their ligands for labeling molecules of interest in vivo. A “target” molecule is linked to a detector molecule (such as a protein or a nucleic acid) and the function and/or location of the target molecule is monitored by binding the detector molecule its ligand, which carries a detectable label. In particular embodiments of the invention, an intracellular fusion protein comprising a target protein and a detector protein is bound to a membrane-permeable, fluorescently-labeled ligand of the detector protein, thereby providing an adjunct or alternative to Green Fluorescent Protein.

REFERENCES:
patent: WO/98/07844 (1998-02-01), None
patent: WO/00/23621 (2000-04-01), None
patent: WO/01/53355 (2001-07-01), None
patent: WO/01/88168 (2001-11-01), None
patent: WO/02/068678 (2002-09-01), None
patent: WO/2004/070351 (2004-08-01), None
Lin et al, Supplementary Info/material for Linja9941532, J. Am. Chem. Soc., 2000, vol. 122: pp. S1-S12.
Baccanari, D. et al., “Effect of a Single Amino Acid Substitution onEscherichia coliDihydrofolate Reductase Catalysis and Ligand Binding”, J. Biol. Chem. 1981: vol. 256: pp. 1738-1747.
Kaufman, R. et al., “The Phosphorylation State of Eucaryotic Initiation Factor 2 Alters Translational Efficiency of Specific mRNAs”, Mol. Cell. Biol., 1989, vol. 9: pp. 946-958.
Keppler et al., 2004, Labeling of fusion proteins with synthetic fluorophores in live cells, Proc. Natl. Acad. Sci. USA, 101(27):9955-9959.
Milleret el., 2004, Methotrexate conjugates: a molecular in vivo protein tag, Angew Chem Int Ed Engl. 43(13):1672-5.
Ishibashi et al., 2003, IL-2 or IL-4 mRNA as a potential flow cytometric marker molecule for selective collection of living T helper 1 or T helper 2 lymphocytes, Anal. Chem., 75, 2715-2723.
Knemeyer et al., 2003, Detection and identification of single molecules in living cells using spectrally resolved fluorescence lifetime imaging microscopy, Anal. Chem., 75, 2147-2153.
Adams et al., 2002, New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications, J. Am. Chem. Soc.124(21):6063-6076.
Bhaumik et al., 2002, Optical imaging of Renilla luciferase reporter gene expression in living mice, Proc. Natl. Acad. Sci. U.S.A. 99:377-382.
Campbell et al., 2002, A monomeric red fluorescent protein, Proc. Natl. Acad. Sci. U.S.A. 99(12):7877-7882.
Chan et al., 2002, Structural studies on bioactive compounds. Part 36: design, synthesis and biological evaluation of pyrimethamine-based antifolates against Pneumocystis carinii, Bioorg. Med/ Chem. 10(9):3001-3010.
Debnath, 2002, Pharmacophore mapping of a series of 2,4-diamino-5-deazapteridine inhibitors of Mycobacterium avium complex dihydrofolate reductase, J. Med. Chem. 45(1): 41-53.
Gilbert, 2002, Inhibitors of dihydrofolate reduces in Leishmania and trypanosomes Biochim Biophys Acta 1587(2-3):249-57.
Molina et al., 2002, A transformed fish cell line expressing a green fluorescent protein-luciferase fusion gene responding to cellular stress, Toxicol. In Vitro 16(2):201-207.
Lau et al., 2001, Efficacies of lipophilic inhibitors of dihydrofolate reductase against parasitic protozoa, Antimicrob. Agents Chemother. 45(1):187-95.
Nagakubo et al., 2001, Characteristics of transport of fluoresceinated methotrexate in rat small intestine, Life Sci. 69(7):739-747.
Ozawa et al., 2001, Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing, Anal. Chem. 73(11):2516-2521.
Subramaniam et al., 2001, Direct visualization of protein interactions in plant cells, Nature Biotechnol. 19(8):769-772.
Suling and Maddry, 2001, Antimycobacterial activity of 1-deaza-7,8-dihydropteridine derivatives against Mycobacterium tuberculosis and Mycobacterium avium complex in vitro J. Antimicrob. Chemother, 47(4):451-454.
Wang et al., 2001, A Study of protein-protein interactions in living cells using luminescence resonance energy transfer (LRET) from Renilla luciferase to Aequorea GFP, Mol. Gen. Genet. 264(5):578-587.
Griffin et al., 2000, Fluorescent labeling of recombinant proteins in living cells with F1AsH, Methods Enzymol. 327:565-578.
Lin et al., 2000, Dexamethasone-Methotrexate: An Efficient Chemical Inducer of Protein Dimerization In Vivo, J. Am. Chem. Soc. 122:4247-4248.
Suling et al., 2000, Antimycobacterial activities of 2,4-diamino-5-deazapteridine derivatives and effects on mycobacterial dihydrofolate reductase, Antimicrob. Agents Chemother. 44:2784-2793.
Famulok et al., 1999, Aptamers as tools in molecular biology and immunology, Curr Top Microbiol Immunol. 243:123-36.
Farinas and Verkman, 1999, Receptor-mediated targeting of fluorescent probes in living cells, J. Biol. Chem. 274:7603-7606.
Karp et al., 1999, A streptavidin-luciferase fusion protein: comparison and applications, Biomol. Eng. 16(1-4):101-104.
Clackson et al, 1998, Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity, Proc. Natl. Acad. Sci. U.S.A. 95:10437-10442.
Clackson, 1998, Redesigning small molecule-protein interfaces, Curr. Opin. Struct. Biol. 8:451-458.
Griffin et al., 1998, Specific covalent labeling of recombinant protein molecules inside live cells, Science 281: 269-272.
Amara et al., 1997, A versatile synthetic dimerizer for the regulation of protein-protein ineractions Proc. Natl. Acad. Sci. U.S.A. 94:10618-10623.
Jolivet et al., 1997, Confocal microscopy visualization of antifolate uptake by the reduced folate carrier in human leukaemic cells, Br J Cancer. 76(6):734-8.
Weiss et al., 1997, RNA aptamers specifically interact with the prion protein PrP J. Virol. 71(11):8790-8797.
Belshaw et al., 1996, Controlling protein association and subcellular localization with a synthetic ligand that induces heterodimerization of proteins, Proc. Natl. Acad. Sci. U.S.A. 93:4604-4607.
Carey et al., 1996, Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear protein import, J. Cell Biol. 133(5):985-986.
Farrar et al., 1996, Activation of the Raf-1 kinase cascade by coumerycin-induced dimerization, Nature 383:178-181.
Heim et al., 1996, Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer, Curr. Biol. 6:178-182.
Htun et al., 1996, Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera, Proc. Natl. Acad. Sci. U.S.A. 93(10):4845-4850.
Knowles et al., 1996, Translocation of RNA granules in living neurons, J. Neurosci. 16, 7812-7820.
Mitra et al., 1996, Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein, Gene 173:13-17.
Ogawa et al., 1995, Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells, Proc. Natl. Acad. Sci. U.S.A. 92:11899-11903.
Banerjee et al., 1994, Transfection with a cDNA encoding a Ser31 or Ser34 mutant human dihydrofolate reductase into Chinese hamster ovary and mouse marrow progenitor cells confers methotrexate resistance, Gene 139(2): 269-274.
Spencer et al., 1993, Controlling signal transduction with synthetic ligands Science, 262:1019-1024.
Assaraf et al., 1992, Characterization of the coexisting multiple mechanisms of methotrexate resistance in mouse 3T6 R50 fibroblasts, J. Biol. Chem. 267:5776-5784.
Trippett et al., 1992, Defective transport as a mechanism of acquired resistance to methotrexate in patients with acute lymphocytoc leukemia, Blood 80:1158-1162.
Fan et al., 1991, Affinity labeling of folate transport proteins with the N-hydroxysuccinimide ester of the gamma-isomer of fluorescein-methotrexate, Biochemistry. 30(18):4573-4580.
Assaraf et al., 1989, A fluorescein-methotrexate-based flow cytometric bioassa

Affiliated with

Also associated with

Rating

Ligand/binding partner bio-labeling systems does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ligand/binding partner bio-labeling systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ligand/binding partner bio-labeling systems will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4093841