Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal
Reexamination Certificate
2004-11-22
2009-06-30
Sajjadi, Fereydoun G (Department: 1633)
Multicellular living organisms and unmodified parts thereof and
Method of making a transgenic nonhuman animal
C800S018000, C435S441000
Reexamination Certificate
active
07554004
ABSTRACT:
The present invention relates to methods of producing an allelic series of modifications in genes of interest in a cell. In particular, the invention provides methods for using nucleic acid sequence-modifying agents (e.g., chemicals, electromagnetic radiation, etc.) to introduce modifications in any nucleic acid sequence in the genome of a cell. Also provided are sets of cells which contain at least one modification in any gene of interest. The methods and compositions of the invention are useful in determining the function of the gene of interest.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4965188 (1990-10-01), Mullis et al.
patent: 6015670 (2000-01-01), Goodfellow
patent: 6033861 (2000-03-01), Schafer et al.
Pious et al. Immunogenetics 4:437-448; 1977.
Moreadith et al., J. Mol. Med., 75:208-216, 1997.
Hochepied et al. Stem Cells 22:441-447; 2004.
Russell et al. (1979) “Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse,” Proc. Natl. Acad. Sci. USA 76:5818-5819.
Hitotsumachi et al. (1985) “Dose-repetition increases the mutagenic effectiveness ofN-ethyl-N-nitrosourea in mouse spermatogonia,” Proc. Natl. Acad. Sci. USA 82:6619-6621.
Shedlovsky et al. (1993) “Mouse Models of Human Pheynlketonuia,” Genetics 134:1205-1210.
Marker et al. (1997) “Spectrum ofBmp5Mutations From Germline Mutagenesis Experiments in Mice,” Genetics 145:435-443.
Zambrowicz et al. (1998) “Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells,” Nature 392:608-611.
Maniatis et al. (1987) “Regulation of Inducible and Tissue-Specific Gene Expression,” Science 236:1237-1245.
Voss et al. (1986) “The role of enhancers in the regulation of cell-type-specific transcriptional control,” Trends Biochem. Sci. 11:287-289.
Dijkema et al. (195) “Cloning and expression of the chromosomal immune interferon gene of the rat,” EMBO J. 4:761-767.
Uetsuki et al. (1989) “Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1a,”J. Biol. Chem. 264:5791-5798.
Lim et al. (1990) “Use of the human elongation factor 1apromoter as a versatile and efficient expression system,” Gene 91:217-223.
Mizushima and Nagata (1990) “pEF-BOS, a pwerful mammalian expression vector,” Nuc. Acids. Res. 18:5322.
Gorman et al. (1982) “The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection,” Proc. natl. Acad. Sci. USA 79:6777-6781.
Boshart et al. (1985) “A Very Strong Enhancer is Located Upstream of an immediate Early Gene of Human Cytomegalovirus,” Cell 41:521-530.
Kohler et al. (1993) “Spectrum of Mutation and Frequency of Allelic Deletion of the p53 Gene in Ovarian Cancer,” J. Natl. Cancer Inst. 85(18):1513-1519.
Greenman et al. (1998) “Identification od Missense and truncation Mutations in theBRCA1Gene in Sporadic and Familial Breat and Ovarian Cancer,” Genes, Chromosomes & Cancer. 21(3):244-249.
Zielenski and Tsui (1995) “Cystic Fibrosis: Genotypic and Phenotypic Variations,” Ann. Rev. Genetics 29:777-807.
Dean and Santis (1994) “Heterogeneity in the severity of cystic fibrosis and the rolse of CFTR gene mutations,” Hum. Genet. 93(4):364-368.
Sessa et al. (1997) “Autosomal dominant polycystic kidney disease: clinical and genetic aspects,” J. Nephrol. 10(6):295-310.
Watnick et al. (1997) “An unusual pattern of mutation in the duplicated portion ofPKD1is revealed by use of a novel strategy for mutation detection,” Human Molec. Genetics 6:1473-1481.
Veldhuisen et al. (1997) “A Spectrum of Mutations in the Second Gene for Autosomal Dominant Polycystic Kidney Disease (PKD2), ” Am. J. Hum. Genet. 61:547-555.
Schulte-Merker et al. (1992) “The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo,” Development 116:1021-1032.
Hermann et al. (1990) “Cloning of theTgene required in mesoderm formation in the maouse,” Nature 343:617-622.
Smith et al. (1990) “Expression of a Xenopus Homolog ofBrachyury(T) Is an Immediate-Early Response to Mesoderm Induction,” Cell 67:79-87.
Blum et al. (1992) “Gastrulation in the Mouse: The Role of the Homeobox Genegoosecoid,” Cell 69:1097-1106.
Izpisua-Belmonte et al. (1993) “The Homeobox Genegoosecoidand the Origin of Organizer Cells in the Early Chick Blastoderm,” Cell 76:645-659.
Blumberg et al. (1991) “Organizer-Specific Homeobox Genes inXenopus laevisEmbryos,” Science 253:194-196.
Stachel et al. (1993) “Lithium perturbation andgoosecoidexpression identify a dorsal specification pathway in the pregastrula zebrafish,” Development 117:1261-1274.
Evans et al. (1981) “Establishment in culture of pluripotential cells from mouse embryos,” Nature 292:154-156.
Martin (1981) “Isloation of a pluripotential cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells,” Proc. Natl. Acad. Sci USA 78:7634-7638.
Magnuson et al. (1982) “The development of monosomy 19 mouse embryos,” J. Embryo. Exp. Morph. 69:223-236.
Doetschmann et al. (1988) “Establishment of Hamster Blastocyst-Derived Embryonic Stem (ES) Cells,” Dev. Biol. 127:224-227.
Tokunaga et al. (1989) “Establishment of the Mouse Embryonic Stem Cell Lines from Whole Blastocysts and Isolated Inner Cell Masses,” Jpn. J. Anim. Reprod. 35:173-178.
Eistetter (1989) “Pluripotential Embryonal Stem Cell Lines Can Be Established from Disaggregated Mouse Morulae,” Dev, Gro. Differ. 31:275-282.
Matsui et al. (1992) “Derivation of Pluripotential Embryonic Stem Cells from Murine Primordial Germ Cells in Culture,” Cell 70:841-847.
Resnick et al. (1992) “Long-term proliferation of mouse primordial germ cells i n culture,” 359:550-551.
Doetschman et al. (1985) “The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium,” J. Embryol. Exp. Morphol. 87:27-45.
Lallermand et al. (1990) “An in situ assessment of the routed and extent of colonisation of the mouse embryo by embryonic stem cells and their descendants,” Development 110:1241-1248.
Bradley et al (1984) “Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines,” Nature 309:255-256.
Gossler et al. (1986) “Transgenesis by means of blastocyst-derived embryonic stem cell lines,” Proc. Natl. Acad. Sci. USA 83:9065-9069.
Robertson et al. (1986) “Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector,” Nature 323:445-448.
Beddington et al (1989) “An assessment of the development potential of embryonic stem cells in the midgestion mouse embryo,” Development 105:733-737.
Suemori et al. (1990) “A mouse embryonic stem cell line showing pluripotency of differentiation in early embryos and ubiquitous β-galactosidase expression,” Cell Differ. Dev. 29:181-186.
Strojeck et al. (1990) “A Method for Cultivating Morphologically Undifferentiated Embryonic Stem Cells from Porcine Blastocysts,” Theriogenology 33:901-914.
Piedrahita et al. (1990) “On the Isolation of Embryonic Stem Cells: Comparative Behavior of Murcine, Porcine and Ovine Embrosm” Theriogenology 34:879-901.
Notarianni et al. (1991) “Derivation of pluripotent, embryonic cell line from the pig and sheep,” J. Reprod. Fert. (Suppl.) 43:255-260.
Saito et al. (1992) “Bovine embryonic stem cell-like lines cultured over several passages,” Roux's Arch.
Avner Ellis D.
Magnuson Terry R.
Woychik Richard P.
Case Western Reserve University
Medlen & Carroll LLP
Sajjadi Fereydoun G
LandOfFree
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