High sensitivity fluorometric assays

Details High sensitivity fluorometric assays High sensitivity fluorometric assays

2004-02-09

2009-06-30

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S014000, C435S069200

Reexamination Certificate

active

07553614

ABSTRACT:
Spectrophotometric assays are rendered more sensitive by adding to the assay mixture a light-emitting moiety. The decrease in intensity of light emitted due to the presence of a light-absorbing moiety associated with the photometric assay is much more sensitive to analyte than the absorbance of the light-absorbing moiety itself.

REFERENCES:
patent: 4277437 (1981-07-01), Maggio
patent: 4318707 (1982-03-01), Litman et al.
patent: 4798788 (1989-01-01), Sands
patent: 4853327 (1989-08-01), Dattagupta
patent: 5173434 (1992-12-01), Morris et al.
patent: 5254477 (1993-10-01), Walt
patent: 5459567 (1995-10-01), Brocia
patent: 5565328 (1996-10-01), Bascomb et al.
patent: 5744320 (1998-04-01), Sherf et al.
patent: 2005/0233401 (2005-10-01), Brocia
patent: 0 171 158 (1986-12-01), None
patent: 0 091 837 (1989-07-01), None
Lakowicz, Joseph Principles of Fluorescence Spectroscopy Plenum Press, NY. 1983, Chapter 9, Quenching of Fluorescence, pp. 257-295.
Ando et al., Analytical Biochemistry (1983) 129:170-175.
Blumberg et al., Clin. Chem. (1980) 26:409-413.
Carmel et al., Eur. J. Biochemistry (1977) 73:617-625.
Carmel et al., Eur. J. Biochemistry (1978) 87:265-273.
Chen, Analytical Letters (1978) B11(3):249-255.
Fleminger et al., Eur. J. Biochem. (1982) 125:609-615.
Florentin et al., Analytical Biochemistry (1984) 141:62-69.
Haugland, Handbook of Fluorescent Probes and Research Chemicals, 6th ed. by Molecular Probes, Eugene, OR.
Yaron et al., Analytical Biochemistry (1979) 95:228-235.

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