Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2003-09-19
2009-06-09
Helms, Larry R. (Department: 1648)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C536S023100, C424S218100
Reexamination Certificate
active
07544775
ABSTRACT:
The present invention relates to a system for expressing toxic proteins, to an expression vector comprising this system, to a prokaryotic cell transformed with this system, and also to a method for synthesizing a toxic protein using this expression system. The expression system of the invention is characterized in that it comprises successively, in the 5′-3′ direction, a nucleotide sequence encoding the Asp-Pro dipeptide and a nucleotide sequence encoding a toxic protein. According to a preferred embodiment of the invention, the expression system also comprises, upstream of the Asp-Pro sequence, a nucleotide sequence encoding a soluble protein. The expression system of the invention makes it possible to construct an expression vector that is useful for transforming a prokaryotic cell such asE. coli, for example in a method for synthesizing the toxic protein.
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Falson Pierre
Montigny Cédric
Penin François
Boesen Agnieszka
Buchanan & Ingersoll & Rooney PC
Centre National de la Recherche Scientifique
Helms Larry R.
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