Polynucleotide encoding 2-hydroxyisoflavanone dehydratase...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase

Reexamination Certificate

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C435S004000, C435S006120, C435S440000, C435S069100, C435S071100, C536S023200

Reexamination Certificate

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07462478

ABSTRACT:
2-Hydroxyisoflavanone dehydratase substantially having the amino acid sequence represented by SEQ ID NO: 1 is isolated from licorice. Further, a polynucleotide encoding 2-hydroxyisoflavanone dehydratase of the SEQ ID NO: 2 is obtained. Furthermore, the amino acid sequence of 2-hydroxyisoflavanone dehydratase is identified from soybean and a polynucleotide encoding 2-hydroxyisoflavanone dehydratase is obtained.

REFERENCES:
Branden et al. Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Akashi et al. Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratas. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis. Plant Physiol. Mar. 2005;137(3):882-91. Epub Feb. 25, 2005.
Akashi T. et al., New Scheme of the Biosynthesis of Formononetin Involving 2, 7, 4′-Trihydroxyisoflavanone but Not Daidzein as the Methyl Acceptor., Biosci. Biotechnol. Biochem. (2000), vol. 64, No. 10, pp. 2276 to 2279.
Akashi, T. et al., Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice., Plant. Physiol., (Nov. 1999), vol. 121, No. 3, pp. 821 to 828.
Akashi, T. et al., cDNA cloning and biochemical charaterization of S-adenosyl-L-methionine: 2,7,4′-trihydroxyisoflavanone 4′-O-methyl transferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway., Plant.Cell Physiol., (Feb. 2003), vol. 44, No. 2, pp. 103 to 112.
Hamanatsuka, T. et al., Purification of 2-hydroxyisoflavonone dehydratase from the cell cultures ofPueraria labata., Phytochemistry (1998), vol. 49, No. 2, pp. 497 to 505.

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