DNA intercalating agents and methods of use

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C536S023100, C536S024300, C536S024330, C536S025300, C536S026600

Reexamination Certificate

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07462468

ABSTRACT:
The present invention provides compositions and methods for detecting incorporation of a labeled nucleotide triphosphate onto the growing end of a primer nucleic acid molecule. The method is used, for example, to genotype and sequence a nucleic acid. In a preferred embodiment, the method described herein detects individual NTP molecules.

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patent: 6664047 (2003-12-01), Haugland et al.
Rye, et al.; Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications; Nucleic Acids Research; Feb. 1992; vol. 20, No. 11, pp. 2803-2812.
Seeman, N.C.; At the Crossroads of Chemistry, Biology and Materials: Structural DNA Nanotechnology; Chemistry & Biology; 2003; vol. 10, 1151-1159.
Choi, Y., et al.; Synthesis and Functional Evaluation of DNA-Assembled Polyamidoamine Dendrimer Clusters for Cancer Cell-Specific Targeting; Chemistry & Biology; 2005; vol. 12, 35-43.

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