Method for screening agent acting on cell wall

Details Method for screening agent acting on cell wall Method for screening agent acting on cell wall

2002-10-22

2008-12-02

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Reexamination Certificate

active

07459268

ABSTRACT:
A method for screening an agent acting on a cell wall, which comprises the following steps of: (1) culturing a microorganism having a reporter protein fixed on a cell wall as a GPI-anchored protein in the presence of a test agent acting on a cell wall; (2) analyzing a saccharide chain of a substance derived from the reporter protein released in a culture fluid of the microorganism; and (3) estimating a targeting site of the test agent on the cell wall on the basis of information of the saccharide chain of the substance derived from the reporter protein obtained in the step (2). An agent acting on a cell wall directed to a particular targeting site can be efficiently screened by conveniently and suitably determining a targeting site of an agent having an inhibitory action on a cell wall.

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