Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2005-06-22
2008-11-04
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
C436S057000
Reexamination Certificate
active
07445909
ABSTRACT:
The present invention is directed to a method for detecting reactive metabolites using a combination of glutathione and a stable isotopic derivative of glutathione and mass spectroscopy. More specifically, the method selectively detects reactive metabolites by eliminating false positives arising from non-reactive components including both unreactive metabolites and components of the reaction mixture.
REFERENCES:
patent: 6197575 (2001-03-01), Griffith et al.
patent: 2002/0034729 (2002-03-01), Avery et al.
patent: 2003/0180962 (2003-09-01), Kaplan et al.
patent: 2005/0186651 (2005-08-01), Gan et al.
patent: 2008/0081349 (2008-04-01), Huebert et al.
patent: 1150120 (2001-10-01), None
patent: WO 91/08762 (1991-06-01), None
patent: WO 03/027682 (2003-04-01), None
VandenHeuvel W. Drug Metabolite Identification: Stable Isotope Methods. J Clinical Pharmacology 26(6)427-434, Jul./Aug. 1986.
Samuel K. et al. Addressing the Metabolic Activation Potential of New Leads in Drug Discovery . . . J of Mass Spectrometry, vol. 38, pp. 211-221, Jan. 2003.
International Search Report PCT/US2005/022121 dated Oct. 28, 2005.
Yan, Zhengyin et al: “Stable-isotope trapping and high-throughput screenings of reactive metabolites using the isotope MS signature.” Analytical Chemistry Dec. 1, 2004, vol. 76, No. 23, pp. 6835-6847 XP002348111.
Pearson P G et al: “Screening strategy for the detection of derivatized glutathione conjugates by tandem mass spectrometry” Analytical Chemistry, American Chemical Society Columbus, US, vol. 62, No. 17, 1990, pp. 1827-1836 XP001203557 ISSN: 0003-2700.
Baillie T A et al: “Mass spectrometry in the analysis of glutathione conjugates” Biological Mass Spectrometry, Wiley and Sons, Chichester, GB, vol. 22, No. 6, 1993, pp. 319-325 XP001016370 ISSN: 1052-9306.
Yan et al, “Stable-Isotope Trapping and High-Throughput Screenings of Reactive Metabolites Using the Isotope MS Signature,” Anal. Chem., 2004, pp. 6835-6847, vol. 76, American Chemical Society, published on the web.
Mutilb et al, “Application of stable isotope labeled glutathione and rapid scanning mass spectrometers in detecting and characterizing reactive metabolites,” Rrapid Commun. Mass Spectrom, 2005, pp. 3482-3492, vol. 19, Rapid Communications in Mass Spectrometry, John Wiley & Sons, Ltd.
Mutlib et al, “Formation of Unusual Glutamate Conjugates of 1-[3-(Aminomethyl) Phenyl]-N-[3-Fluoro-2′-(Methylsulfonyl)-1[1,1′-Biphenyl]-4-YL]3-(Trifuloromethyl)-1H-Pyrazole-5-Carboxamide (DPC 423) and Its Analogs: The role of γ-Glutamyltranspeptidase in the Biotransformation of Benzylamines,” Drug Metabolism and Disposition, 2001, pp. 1296-1306, vol. 29, No. 10, The American Society for Pharmacology and Experimental Therapeutics, USA.
Borman et al, “Nipping Bad Drugs in the Bud,” C&EN On line, 2004, pp. 36-39.
Evans et al, “Drug-Protein Adducts: An Industry Perspective on Minimizing the Potential for Drug Bioactivation in Drug Discovery and Development,” Chem Res. Toxicol., 2004, pp. 3-16, vol. 17, American Chemical Society.
Gan et al, “Dansyl Glutathione as a Trapping Agent for the Quantitative Estimation and Identification of Reactive Metabolites,” Chem Res. Toxicol., 2005, pp. 896-903, vol. 18, American Chemical Society.
Adang et al, “The glutathione-binding site in glutathione S-transferases,” Investigation of the cysteinyl, glycyl and γ-glutamyl domains, J Biochem, 1990, pp. 47-54, vol. 269, Great Britain.
Caldwell Gary W.
Yan Zhengyin
Gitomer Ralph
Janssen Phamaceutica N.V.
Stercho Yuriy
LandOfFree
Method for detecting reactive metabolites using a... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detecting reactive metabolites using a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting reactive metabolites using a... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4040494