Method and device for recognizing dark states during the...

Details Method and device for recognizing dark states during the... Method and device for recognizing dark states during the...

2006-02-21

2008-11-04

Hannaher, Constantine (Department: 2884)

Radiant energy

Luminophor irradiation

Methods

Reexamination Certificate

active

07446322

ABSTRACT:
A method for recognizing dark states during the spectroscopic or microscopic examination of fluorescent specimens includes varying an intensity distribution of excitation light by varying an excitation/illumination volume over a plurality of mutually independent measurements. A determination is made as to whether observed time constants change between the measurements. The existence of a dark state is inferred where the observed time constants are unchanged between the measurements.

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